新疆南疆牛病毒性腹泻病毒基因型鉴定及分析  

Genotype identification of bovine viral diarrhea virus in southern Xinjiang

作　　者：王青青[1] 张迎春[2] 崔鑫[1] 胡建军[1] 彭安业[2] 王娜[1] 张婉琪[3] 张莹钰[3] WANG Qingqing;ZHANG Yingchun;CUI Xin;HU Jianjun;PENG Anye;WANG Na;ZHANG Wanqi;ZHANG Yingyu(College of Animal Sciences, Tarim University, Alar 843300, China;The Veterinary Station of the First Division, Xinjiang Production and Construction Group, Alar 843300, China;College of Life Sciences, Tarim University, Alar 843300, China)

机构地区：[1]塔里木大学动物科学学院,阿拉尔843300 [2]新疆生产建设兵团第一师畜牧兽医工作站,阿拉尔843300 [3]塔里木大学生命科学学院,阿拉尔843300

出　　处：《畜牧与兽医》2018年第7期113-117,共5页Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31660725,31160497);新疆生产建设兵团第一师科技局资助项目(2016XS03);新疆生产建设兵团科技局现代农业科技攻关与成果转化资助项目(2016AC012);中国兽医现场流行病学培训项目(CFETPV)

摘　　要：为掌握新疆南疆地区规模奶牛场牛病毒性腹泻病毒（BVDV）感染的病毒基因类型,更好地采取防控措施,本研究以BVDV基因高度保守的5＇-UTR核苷酸序列设计并合成通用引物,提取疑似感染BVDV样品的核酸,采用一步法RT-PCR,扩增获得目的基因片段,经测序、比对、核苷酸同源性比较及构建基因遗传进化树。结果显示,30份疑似样品中,有2份样品PCR扩增阳性;测序分析显示,所检的阳性样品均为BVDV Ic型。研究表明,所调查的新疆南疆规模化奶牛场存在BVDV Ic型的感染,证实新疆地区牛场BVDV基因型存在多样性,为新疆规模奶牛场BVD病原鉴定、防控提供了理论依据。This study was to identify the genotype of bovine viral diarrhea virus on the large-scale cattle farms in southern Xinjiang. Specific primers were designed according to the published sequence of bovine viral diarrhea virus 5＇-UTR gene. RNA samples were extracted and detected by RT-PCR with the special primers. The amplified fragments were sequenced. Analysis of the nucleotide sequences and construction of a phylogenetic tree were based on the 5＇-UTR gene of bovine viral diarrhea virus with a biological software. The sequencing results showed that all the positive samples tested were of the BVDV Ic genotype,indicating BVDV Ic infection on large-scale cattle farms in southern Xinjiang,and that there were diverse genotypes of BVDVs there. The findings here provide theoretical basis for prevention and control of BVD pathogens on large-scale cattle farms in southern Xinjiang.

关 键 词：BVDV 5'-UTR基因 序列分析 

分 类 号：S855.3[农业科学—临床兽医学]