生长分化因子11对糖尿病大鼠内皮祖细胞数量和功能的影响  

Effects of growth differentiation factor 11 on the amount and function of endothelial progenitor cells in diabetic rats

作　　者：张佳佳[1] 向光大[1] 李欢[1] 朱彪[1] 王力 郭孛 向林[1] 董靖[1] 刘敏[1] Zhang Jiajia;Xiang Guangda;Li Huan;Zhu Biao;Wang Li;Guo Bei;Xiang Lin;Dong Jing;Liu Min(Department of Endocrinology, Wuhan General Hospital of Guangzhou Military, Wuhan 430070, Chin)

机构地区：[1]广州军区武汉总医院内分泌科,武汉430070

出　　处：《国际内分泌代谢杂志》2018年第4期233-236,F0003,共5页International Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金（81370896、81570730）

摘　　要：目的探讨生长分化因子11（GDF11）对糖尿病大鼠内皮祖细胞（EPC）数量及功能的影响。方法雄性Sprague-Dawley大鼠40只按随机数字法随机分为正常对照组（n=10）和糖尿病组（n=30）。糖尿病组大鼠通过腹腔注射链脲佐菌素（STZ）建立糖尿病大鼠模型。建模12周后，取20只糖尿病大鼠按照随机数字法分为模型对照组与实验组（每组10只），实验组腹腔注射重组人GDF11蛋白0．1mg／kg，连续干预14d，模型对照组给予等量磷酸盐缓冲液。14d后，取腹主动脉血，利用流式细胞仪检测EPC的数量，取大鼠股骨及胫骨骨髓，分离培养EPC，通过小管形成实验及迁移实验检测EPC的功能。结果与正常对照组相比，模型对照组大鼠循环EPC数量明显减少（t=4．823，P〈0．01），迁移能力及小管形成能力亦降低（t=-15．236、-7．005，P均〈0．01）。与模型对照组相比，实验组大鼠循环EPC数量明显增多（t=2．784，P〈0．05），同时迁移能力及小管形成能力提高（t=-7．066、-6．296，P均〈0．01）。结论GDF11可以动员糖尿病大鼠循环EPC，增强其EPC的迁移和小管形成能力。Objective To investigate the effects of growth differentiation factor 11 （ GDF11 ） on the amount and function of endothelial progenitor cell （ EPC ） in diabetic rats. Methods Forty male Sprague- Dawley （SD）rats were randomly divided into normal control group （n = 10 ） and diabetic group （n = 30 ） ac- cording to random number method. All rats in diabetic group were injected with streptozotocin to establish di- abetes model. After 12 weeks of modeling, 20 diabetic rats were then randomly divided into vehicle control group and experimental group according to random number method. Rats in experimental group were intrap- eritoneally injected with recombinant human GDF11 protein O. 1 mg/kg for 14 days, and rats in vehicle con- trol group were treated with an equivalent volume of phosphate buffered saline. After 14 days, the blood from abdominal aorta were collected to detect the number of EPC by flow cytometry. EPCs from the femur and tibi- a bone marrow were isolated to assess the migration and tube formation function of EPCs by Transwell cham- ber assay. Results Compared with normal control group, both the number of EPC （ t = 4. 823, P 〈 0. 01 ） and the migration and tube formation ability were decreased （ t = - 15. 236, - 7. 005, all P 〈 0.01 ） in vehi- cle control group. Compared with vehicle control group, the number of EPC （ t = 2. 784, P 〈 0.05 ） as well as the migration ability and tube formation ability were increased in experimental group （t = -7. 066, -6. 296, all P 〈 0. 01 ）. Conclusion GDF11 can mobilize peripheral blood EPC in diabetic rats and en- hance the migration and tube formation abilities of EPC.

关 键 词：生长分化因子11 糖尿病 内皮祖细胞 

分 类 号：R587.2[医药卫生—内分泌]