小鼠免疫性冠状动脉炎内皮细胞线粒体的动态变化  

The Dynamic Changes of Mitochondria in Endothelial Cells of Immunological Coronaritis in Mice

作　　者：濮祥强 王祥 钱光辉 马锦[1] 丁粤粤[1] 吕海涛[1] PU Xiang-qiang;WANG Xiang;QIANG Guang-hui;MA Jin;DING Yue-yue;LV Hai-tao(Children＇s Hospital of Soochow University, Suzhou 215025, China;Pediatric Institute of Soochow University, Suzhou 215025, China;HuaL An First People＇s Hospital, Huai, An 223300, China)

机构地区：[1]苏州大学附属儿童医院,苏州215025 [2]淮安市第一人民医院,淮安223300 [3]苏州大学儿科临床研究院,苏州215025

出　　处：《实验动物与比较医学》2018年第3期169-175,共7页Laboratory Animal and Comparative Medicine

基　　金：国家自然科学基金(81370217、81570455、81400222、81300124)

摘　　要：目的初步观察小鼠免疫性冠状动脉炎血管内皮细胞线粒体形态的动态变化,以模拟川崎病(KD)冠状动脉炎相应的内皮细胞及线粒体改变。方法 C57BL/6小鼠随机分为实验组(n=30)和对照组(n=30)。分别连续5 d予腹腔注射白色念珠菌水溶物或磷酸盐缓冲液(PBS)。末次注射后24 h、3 d、7 d、14 d及28 d,每组取6只小鼠处死,取血检测部分血常规及血浆中白细胞介素6(IL-6)及肿瘤坏死因子α(TNF-α)水平。心脏及冠状动脉标本经苏木精-伊红(HE)染色后行组织病理学检查。透射电子显微镜观察冠状动脉内皮细胞线粒体结构。结果实验组小鼠血白细胞(WBC)、血小板(PLT)值,血浆IL-6、TNF-α水平,在注射后3 d、7 d时升高(P均<0.05)。冠状动脉组织病理学在初期(3 d)表现为内皮细胞肿胀,中期(14 d)后出现弹力纤维断裂、血管瘤形成。冠状动脉内皮细胞线粒体透射电子显微镜下在初中期(24 h、3 d、7 d、14 d)表现为肿胀、空泡样变,数量减少,嵴模糊,电子密度降低。结论构建的小鼠模型能够模拟出与人KD相似的免疫性冠状动脉炎特征,提示内皮细胞线粒体参与免疫性冠状动脉炎过程。Objective To observe the dynamic changes of morphology of vascular endothelial cell mitochondria in mice with immunologic coronaritis, in order to simulate the corresponding changes in endothelial cells and mitochondrial with coronaritis of Kawasaki disease. Methods C57BL/6 mice were randomly divided into experimental group（n=30） and control group（n=30）. Mice were peritonealy injected with Candida albicans water soluble fraction or phosphate buffer（PBS） for 5 d. After the last injection of 24 h, 3 d, 7 d, 14 d and 28 d, 6 mice in each group were sacrificed to detect the blood routine and the level of interleukin（IL）-6 and tumor necrosis factor（TNF）-α in the plasma. Cardiac and coronary artery specimens were examined by hematoxylin eosin（HE） staining. The structure of mitochondria in the endothelial cells of coronary artery was observed by electron microscopy. Results The levels of blood white blood cells, platelets, plasma IL-6 and TNF-α in the experimental group were increased at the time of 3 d and 7 d after injection（P〈0.05）. The histopathology of the early stage in coronary artery inflammation（3 d）, the endothelial cells were swollen. The fracture of elastic fiber and hemangioma appeared after the mid-term（14 d）. Under the perspective of electron microscopy, coronary artery endothelial cells showed swelling and vacuolar degeneration at middle stage（24 h, 3 d, 7 d and 14 d）, and the number of mitochondrion decreased, the crest blurred, and the electron density decreased.Conclusion The mouse model constructed can simulate characteristics similar to Kawasaki disease immunity coronaritis, suggesting that mitochondria in the endothelial cells involved in the immune process of coronary arteritis.

关 键 词：川崎病(KD) 冠状动脉炎 内皮细胞 线粒体 小鼠 

分 类 号：R729[医药卫生—儿科] Q95-33[医药卫生—临床医学]