隐球菌脑膜炎患者外周血单个核细胞PILRα升高及意义  

Increasing Expression of PILRα in Peripheral Blood Mononuclear Cells from Patients with Cryptococcal Meningitis and Its Significance

作　　者：李腾达[1] 刘鹏[1] 龙曙萍 刘云[1] 黄元兰[2] 张薇薇[1] 郭杰[1] 谷明莉[1] 邓安梅[1] LI Teng-da;LIU Peng;LONG Shu-ping;LIU Yun;HUANG Yuan-lan;ZHANG Wei-wei;GUO Jie;GU Ming-li;DENG An-mei(Changhai Hospital Affiliated to Second Military Medical University, Shanghai 200433 ,China;No. 455 Hospital of PLA , Shanghai 200052, China)

机构地区：[1]第二军医大学长海医院,上海200433 [2]解放军455医院,上海200052

出　　处：《现代检验医学杂志》2018年第3期11-13,共3页Journal of Modern Laboratory Medicine

基　　金：973计划(2013CB531606);国家自然科学基金(81671556;81601406;81471605;81501397;31500721;81501398;81401358;81302579;81273282;81202353);上海申康基金(SHDC22014014);上海教育科学基金(D14017);军队科研基金(BWS14J023;15ZD009;15XD007);美捷登基金(MJR20150019)

摘　　要：目的探究Ⅱ型成对免疫球蛋白样受体(paired immunogobin-like type 2ceceptor alpha,PILRα)在隐球菌脑膜炎患者外周血单个核细胞(peripheral blood mononuclear cells,PBMCs)中的表达情况,初步讨论其临床意义。方法收集2014年5月~2016年11月到长海医院就诊的30例隐球菌脑膜炎患者外周血作为实验组,诊断标准为脑脊液染色新生隐球菌阳性或培养阳性,同期体检的30例健康人外周血作为对照组。以密度梯度离心法获得标本PBMCs,以PCR检测PBMCs中PILRα的基因表达情况,以ELISA法检测血浆中PILRα及相关细胞因子的表达情况。以独立样本t检验进行两组间变量的比较,以Pearson相关系数表示两变量间的相关性。结果实验组与对照组PBMCs中PILRα相对mRNA水平为2.49±0.74vs 1.77±0.69(t=3.898,P=0.000 3),血浆中PILRα的蛋白表达水平为33.81±10.21ng/ml vs 20.01±6.74ng/ml,差异均具有统计学意义(t=6.178,P<0.000 1)。实验组血浆中PILRα的蛋白表达量与IL-1,IL-6和IFN-γ呈正比(r=0.456,0.521,0.467,P<0.05),差异具有统计学意义。结论 PILRα可能参与了隐球菌脑膜炎的免疫炎症过程,是该病潜在的监测标志物和治疗靶点。Objective To explore the expression of paired immunogobin-like type 2 ceceptor alpha（PILRa） in peripheral blood mononuclear cells（PBMCs） from patients with cryptococcal meningitis,primarily discussed its significance. Methods Pe- ripheral blood samples from 30 patients receiving a diagnosis of eryptococcal meningitis in Changhai Hospital from May 2014 to November 2016 was regarded as the experimental subjects,and the diagnosis standardization was that the cerebrospinal fluid dyeing was positive or the culturing was positive, while the specimens from 30 health individuals examined at the same time were classified as the control. PBMCs was separated by density gradient eentrifugation,PCR was used to test the level of PILRa in PBMCs,the expression of PILRa and the related cytokines in plasm was examined by ELISA. Two independent samples＇ t test was used to compare the varies between the two groups. Pearson correlation coefficient was to show the co- relationship for the two measurement data. Results The relative mRNA expression levels of PILRα in PBMCs in experimen- tal and control groups were 2.49±0.74 vs 1.77±0.69 0=3. 898,P=0. 000 3） ,and the protein expression level of PILRα in plasma was 33.81 ± 10.21 ng/ml vs 20.01 ± 6.74 ng/ml （t= 6. 178, P〈0. 000 1 ）, both with statistical significance. In ex- perimental group, the protein expression of PILRα in plasma was positively related with IL-1, IL-6 and IFN-γ,and there was statistical significance （r=0. 456,0. 521,0. 467,P〈0.05）. Conclusion PILRα may participate in the immune-inflammatory process in cryptoeoccal meningitis,and can be the potential biomarker for disease surveillance and the therapeutic target.

关 键 词：隐球菌脑膜炎 Ⅱ型成对免疫球蛋白样受体 白介素-1 白介素-6 干扰素-Γ 

分 类 号：R519.4[医药卫生—内科学] R392.11[医药卫生—临床医学]