中重度颅脑损伤并发脑梗死患者血清miRNA-124与miRNA-210表达的临床意义  

Clinical Application in Detecting Serum microRNA-124 and microRNA-210 in the Moderately Severe Craniocerebral Injury Complicated with the Traumatic Cerebral Infarction

作　　者：程永红[1] 肖小平 CHENG Yong-hong;XIAO Xiao-ping(Department of Clinical Laboratory the Central Hospital of Shangluo City,Shaanxi Shangluo 726000,China;Department of Clinical Laboratory, the Eighth Hospital of Xi ＇an City, Xi＇an 710061, China)

机构地区：[1]商洛市中心医院检验科,陕西商洛726000 [2]西安市第八医院检验科,西安710061

出　　处：《现代检验医学杂志》2018年第3期83-87,共5页Journal of Modern Laboratory Medicine

摘　　要：目的探讨血清微小RNA(microRNA,miRNA)-124和miRNA-210表达在中重度颅脑损伤并发外伤性脑梗死中的临床应用价值。方法收集商洛市中心医院和西安市第八医院住院治疗的57例中重度颅脑损伤患者的血清及临床资料,依据是否发生外伤性脑梗死分为梗死组22例,未梗死组35例,并设立对照组(24例),采用实时荧光定量聚合酶链反应(Real-time-PCR,RT-PCR)技术检测血清miRNA-124和miRNA-210的表达。比较分析血清miRNA-124和miRNA-210的变化与外伤性脑梗死的关系。结果血清miRNA-124和miRNA-210在梗死组、未梗死组和对照组的表达分别为(0.90±0.18,0.39±0.07,0.18±0.03)和(0.95±0.20,0.42±0.10,0.21±0.04)。与对照组比较,血清miRNA-124和miRNA-210在未梗死组和梗死组的表达显著上调,差异均有统计学意义(t=22.99,23.67,均P<0.01;t=35.37,36.93,均P<0.01)。两标志物在梗死组的表达显著高于未梗死组,差异均有统计学意义(t=23.27,25.12,均P<0.01)。大脑前动脉(ACA)、大脑中动脉(MCA)及大脑后动脉(PCA)在梗死组、未梗死组和对照组的平均流速分别为(36.9±7.6,45.3±8.6,33.9±5.5)cm/s,(60.3±7.9,79.2±12.7,49.6±7.2)cm/s,(51.3±7.7,70.5±11.9,43.1±6.9)cm/s.ACA,MCA及PCA在未梗死组的平均流速与对照组比较明显增快,差异有统计学意义(t=12.23,13.10,13.16,均P<0.01)。ACA,MCA和PCA在梗死组的平均流速分别与未梗死组及对照组比较明显减慢,差异有统计学意义[(t=11.30,12.67,12.09,均P<0.01),(t=11.37,11.52,11.09,均P<0.01)]。miRNA-124和miRNA-210在梗死组的表达具有正相关性(r=0.629,均P<0.01),它们分别与梗死组ACA,MCA和PCA的平均流速具有负相关性[(r=-0.653,-0.620,-0.613,均P<0.01),(r=-0.679,-0.656,-0.637,均P<0.01)],而与未梗死组ACA,MCA和PCA的平均流速则具有正相关性[(r=0.640,0.596,0.607,均P<0.01),(r=0.676,0.625,0.638,均P<0.01)]。结论检测血清miRNA-124和miRNA-210的表达可预测外伤性脑梗死的发生及判断其危险程度,估计病�Objective To investigate the clinical application value of the levels of serum miRNA-124 and miRNA-210 in the moderately severe craniocerebral injury complicated with the traumatic cerebral infarction. Methods The serum and clinical data in 57 cases of patients with the moderately severe craniocerebral injury from the Central Hospital of Shangluo City and the Eighth Hospital of Xi＇an City were collected. Patients were divided into the infarction group （n=22） and non infarction group （n= 35） in accordance with whether there happaned the traumatic cerebral infarction,and set up the control group （24 cases in control group）, using the real-time fluorescent quantitative PCR （Real-time-PCR, RT-PCR） technology to detect the expression of serum miRNA-124 and miRNA-210 in comparative analysing the relationship between the changes of serum miRNA-124,-210 and the traumatic cerebral infarction. Results In the infarcted group, non-infarcted group and control group,the expression of miRNA-124 were 0.90±0.18,0.39±0.07 and 0.18 ± 0. 03 , which of miRNA-210 were 0.95± 0. 20,0. 42±0.10 and 0. 21±0.04. The expression of serum miRNA-124 and miRNA -210 in the the infarcted group and non-infarcted group significantly increased compared with those in the control group,the differences had statistical signifi- eances （t=22.99,23.67,all P〈0.01 and t=35.37,36.93,all P〈0.01）. The expression of the two markers were higher in the infarction group than those in the non-infarction group （t= 23.27,25.12 ,all P〈0.01）. In the infarction group, non in-farction group and the control group, the average velocity of the anterior cerebral artery （ACA）, middle cerebral artery （MCA） and posterior cerebral artery （PCA） ,respectively,were （36.9±7.6,60.3±7.9,51.3±7.7） cm/s, （45.3±8.6, 79.2±12.7,70.5±11.9） cm/s. （33.9±5.5,49.6±7.2,43.1±6.9） cm/s. The average velocity of MCA,PCA and ACA was obviously increased in non infarction group compared with those in the control group,and the differe

关 键 词：颅脑损伤 外伤性脑梗死 miRNA-124 miRNA-210 

分 类 号：R651.15[医药卫生—外科学] R392.11[医药卫生—临床医学]