痛风宁对急性痛风性关节炎抗炎作用的时效性研究  

Effect of Chronergy of Tongfengning on Anti-inflammatory in Model Rats with Acute Gouty Arthritis

作　　者：朱亚菊 黄露露[1] 蔡唐彦 王建辉 李保林 赖兴泉 张艺强[1] 滕方舟 郭洁梅 苏友新[1,2] ZHU Yaju;HUANG Lulu;CAI Tangyan;WANG Jianhui;LI Baolin;LAI Xingquan;ZHANG Yiqiang;TENG Fangzhou;GUO Jiemei;SU Youxin(Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China;Fujian Health College,Fuzhou 350101 ,China.)

机构地区：[1]福建中医药大学,福州350122 [2]福建卫生职业技术学院

出　　处：《中国中医骨伤科杂志》2018年第7期1-6,共6页Chinese Journal of Traditional Medical Traumatology & Orthopedics

基　　金：国家自然科学基金资助项目(81473495)

摘　　要：目的:从炎症指标的变化探讨痛风宁对急性痛风性关节炎(AGA)模型大鼠抗炎作用的时效性。方法:将SD大鼠分成正常大鼠与造模大鼠,造模大鼠采用踝关节腔注射尿酸钠(MSU)混悬液建立AGA大鼠模型,正常大鼠同部位注射生理盐水。于造模后4h模型鉴定成功后随机分成秋水仙碱组、痛风宁组、模型组和正常组,每组18只。秋水仙碱组及痛风宁组分别予相应药物灌胃,模型组与正常组同步予生理盐水灌胃,2次/d,共3d.在造模后24h,48h及72h三个时间点,每组各随机选6只取材。观察并检测各组各时间点关节液白细胞数、滑膜组织病理改变、关节液中炎症相关细胞因子IL-1β,TNF-α,PGE2及TGF-β1的含量。结果:痛风宁能降低关节液白细胞数、改善病变关节滑膜组织的病理形态及降低IL-1β,TNF-α及PGE2含量,同时可升高TGF-β1含量,各时间点与模型组相比差异均有统计学意义(P<0.01或P<0.05),且在48h对各项指标的改善作用最明显,优于24h和72h两个时间点,差异有统计学意义(P<0.01或P<0.05)。结论:痛风宁对AGA有明显的抗炎作用,其作用机理可能是通过升高TGF-β1含量,抑制促炎性细胞因子IL-1β,TNF-α和PGE2的释放,减少关节液中白细胞数及改善关节滑膜组织病理变化,从而达到对AGA的抗炎作用,且在用药后48h效果最佳。objective：To observe the changes of inflammatory aspects and to investigate the effect of chronergy of Tongfengning on anti-inflammatory in model rats with acute gouty arthritis（AGA）.Methods：SD rats were divided into normal rats and model rats.The model rats with AGA were established by injection of sodium urmonosodium urate（MSU）lens into ankle joint,while the normal rats were injected with physiological saline at the same site.After the successful model identification at 4 hafter modeling,the rats were randomly divided into colchicine group,Tongfengning group,model group and normal group with 18 rats in each group.The treatment group was given the corresponding drugs.The model group and the normal group were given the same amount of physiological saline intervention,2 times per day for 3 d.In the all groups,6 rats were randomly selected at three time points（24 h,48 hand 72 h）.The number of white blood cells in the joint fluid and the pathological changes of synovial tissue and the contents of inflammatory cytokines（IL-1β,TNF-α,PGE2 and TGF-β1）in articular fluid were observed and detected in each group.Results：Tongfengning could decrease the number of white blood cell in joint fluid,improve the pathological morphology of synovial tissue and decrease the content of IL-1β,TNF-α,PGE2 and increase the content of TGF-β1.Compared with the model group,there were significant difference at each time point（P〈0.01 or P〈0.05）,and the improvement of each index was the most obvious at 48 h,which was better than that at 24 hor 72 h（P〈0.01 or P〈0.05）.Conclusion：Tongfengning has obvious anti-inflammatory effect on AGA.The mechanism may be that Tongfengning can increase the content of TGF-β1,inhibit the release of pro-inflammatory cytokine IL-1β,TNF-α,PGE2 and reduce the number of white blood cells in articular fluid.So,the pathological changes of synovial tissue can be improved and the anti-inflammatory effect on AGA can be achieved.The best effect was at 48 h after administration.

关 键 词：急性痛风性关节炎 痛风宁 炎症相关细胞因子 时效 

分 类 号：R33[医药卫生—人体生理学]