组织工程技术再生卵巢的初步研究  

A Pilot Study of Ovarian Tissue Engineering

作　　者：杨如镜[1,2] 潘婷 林丽娜[1] 黄晓帆[1] 吴淑卿[1] 王兴玲[2] YANG Rujing;PAN Ting;LIN Lina(Shantou Central Hospital,Shantou 505131,China)

机构地区：[1]广东省汕头市中心医院,广东汕头505131 [2]郑州大学第三附属医院

出　　处：《中国医学创新》2018年第10期17-20,共4页Medical Innovation of China

基　　金：汕头市科学技术局(汕市财教[2013]244号)

摘　　要：目的:构建生物材料支架-卵巢细胞的移植体,观察动物体内移植后移植体的结局。方法:酶消化分离卵巢细胞,用细胞示踪剂CellTracker^(TM) CM-DiI标记,体外观察荧光标记细胞的动态变化;卵巢分离的细胞直接行荧光标记,种植于含孔隙的生物材料胶原海绵中,短时培养形成细胞-支架移植体,移植到小鼠背部皮下。1、3周收获移植体,分别进行冰冻和石蜡切片染色观察。结果:细胞荧光示踪剂CM-DiI标记卵巢细胞效率较高,24 h观察可达98.3%;1周后为66.4%,镜下荧光信号明亮,可作为卵巢细胞体内示踪的标记物。卵巢细胞-生物支架移植体移植后1、3周,移植卵巢细胞可在生物支架内存活,支架孔隙内可见大量细胞生长,并有类似血管样结构形成。结论:生物材料胶原海绵支持卵巢来源细胞体内生长,可作为构建三维人工卵巢的生物材料。Objective：To construct a biomaterial scaffold-ovarian cell graft and observe the outcome of the transplanted grafts in vivo.Method：Enzyme digestion and separation of ovarian cells,with cell tracer CellTracker^（TM） CM-DiI labeled in vitro observed fluorescence-labeled cells in the dynamic changes.Ovarian cells isolated directly fluorescent markers,planted in the pore-containing organisms Material collagen sponge,short-term culture to form cell-scaffold grafts,transplanted into the back of the mouse subcutaneous. The grafts were harvested at weeks 1 and 3,frozen and paraffin sections were stained for staining.Result：Cell fluorescence tracer CM-DiI marked high efficiency of ovarian cells,24 hours observed up to 98.3%;66.4% after a week, microscopic fluorescence signal bright,can be used as ovarian cells tracer Mark.Ovarian cell-biological scaffolds were transplanted 1 week and 3 weeks after transplantation,ovarian cells can survive in biological scaffolds, a large number of cell growth can be seen in the scaffold pores, and similar vascular-like structure formation.Conclusion：The biomaterial collagen sponge supports ovarian cells to grow in vivo,which can be used as biomaterials for constructing three-dimensional artificial ovaries.

关 键 词：组织工程 人工卵巢 细胞示踪剂 卵巢细胞 

分 类 号：R318.08[医药卫生—生物医学工程] R71[医药卫生—基础医学]