锤状中鼓藻培养条件的优化  

Optimization of cultural conditions of Bellerochea malleus

作　　者：王永强[1] 王珺[1] WANG Yong-qiang;WANG Jun(Oceanographic College of Hainan University/ Key Laboratory of Tropical Biological Resources,Ministry of Education/ National Demonstration Center for Experimental Marine Biology Education,Hainan University,Haikou 570228,Chin)

机构地区：[1]海南大学海洋学院/热带生物资源教育部重点试验室/海南大学海洋生物试验教学中心,海南海口570228

出　　处：《广东农业科学》2018年第4期140-145,共6页Guangdong Agricultural Sciences

基　　金：海南省重点研发计划项目(ZDYF2016087);国家星火计划项目(2015GA800003)

摘　　要：通过单因子和正交试验,研究了温度、光照、盐度等生态因子及氮、磷、铁、硅、钼、维生素B1、维生素B12和维生素H等营养元素对锤状中鼓藻[Bellerochea malleus(Bright-well)Van Heurek]生长繁殖的影响。结果表明:锤状中鼓藻生长的适宜温度为15~35℃,最适温度为30℃;适宜光照为1 000~8 000 lx,最适光照为4 000 lx;适宜盐度为5~50,最适盐度为35。筛选出锤状中鼓藻的培养液配方为每升天然海水中加入NaNO_3-N 20.0 mg、NaH_2PO_4-P 1.0 mg、FeSO_4-Fe 0.1 mg、Na_2SiO_3-Si 15.0 mg、Na2Mo O4 0.02 mg、VB1 1.0 mg和VH 0.05 mg。The paper studied effects of temperature,illumination intensity,salinity and nutrients on the growth and reproduction of Bellerochea malleus by one-factor experiment and orthogonal experiments.Results showed that the suitable water temperature was 15-35℃ and the optimal water temperature was 30℃.The suitable and optimal illumination intensity were respectively 1 000-8 000 lx and 4 000 lx.The suitable salinity was 5-50 and the optimal salinity was 35.The optimal prescription for culture solution was as follows ：NaNO3-N 20.0 mg/L,NaH2PO4-P 1.0 mg/L,FeSO4-Fe 0.1 mg/L,Na2SiO3-Si 15.0 mg/L,Na2 MoO4 0.02 mg/L,VB1 1.0 mg/L and VH 0.05 mg/L.

关 键 词：锤状中鼓藻 生态因子 营养盐 生长 

分 类 号：Q949.270.5[生物学—植物学]