黄芩素对喉癌细胞周期、凋亡率及细胞核DNA的影响  

Effect of Baicalein on Cell Cycle,Apoptosis Rate,DNA of Apoptotic Cells in Laryngeal Cancer Cells

作　　者：孙吉凤[1] 刘剑凯[2] SUN Jifeng;LIU Jiankai(Changchun Medicial College,Changchun 130031,China)

机构地区：[1]长春医学高等专科学校,吉林长春130031 [2]吉林大学基础医学院

出　　处：《中国医学创新》2018年第16期13-16,共4页Medical Innovation of China

基　　金：吉林省教育厅"十二五"科学技术研究项目资助课题(2014-415)

摘　　要：目的:探索黄芩素(baicalein,BAI)对喉癌细胞周期、凋亡率及细胞核DNA的影响。方法:体外培养的Hep-2细胞,加入不同浓度的BAI,药物作用后,用MTT法检测细胞增殖情况;用流式细胞术检测对细胞周期及细胞凋亡率的影响;用DNA琼脂糖凝胶电泳法检测对凋亡细胞核DNA的影响。结果:MTT法检测到BAI(10、20、40、80μmol/L)可明显抑制Hep-2细胞的增殖,呈时间-剂量依赖性(P<0.05);FCM法检测到BAI(40μmol/L)可诱使细胞阻滞在S期,凋亡率升高(P<0.05);BAI(40、80μmol/L)可诱使细胞核DNA形成"梯状"条带;呈时间-剂量依赖性(P<0.05)。结论:一定浓度的BAI,可抑制人喉癌Hep-2细胞增殖,诱导细胞阻滞在S期,细胞凋亡率升高,晚期凋亡细胞核DNA断裂形成DNA Ladder。Objective：To investigate the effect of Baicalein（BAI） on cell cycle,apoptosis rate,DNA of apoptotic cells in laryngealcancer cells.Method：Hep-2 cells were exposed to BAI at various concentrations respectively,in vitro.MTT assay was used to determine cell proliferation;flow cytometry were used to analyze cell cycle and apoptosis rate;the effect on the DNA was detected by agarose gel electrophoresis.Result：MTT assay showed that the proliferation of Hep-2 cells were significantly inhibited by BAI（10,20,40 and 80 μmol/L）,in a time and dose dependent manner（P〈0.05）.Hep-2 cells are arrested at the S phase and the apoptosis rate was significantly increased by BAI（40 μmol/L）through flow cytometry（P〈0.05）.DNA ladder in Hep-2 cells was induced by BAI（40,80 μmol/L）.Conclusion：BAI can inhibite proliferation of Hep-2 cells,increase the apoptosis rate,induce Hep-2 cells in S phase and late apoptotic cells DNA broken into DNA Ladder.

关 键 词：黄芩素 喉癌 细胞周期 凋亡率 

分 类 号：R285.5[医药卫生—中药学]