HPLC法同时测定壮药华陀方中绿原酸、异嗪皮啶和迷迭香酸的含量  

Simultaneous Detemrination of Chlorogenic Acid,Isofraxidin and Rosmarinic Acid in Zhuang Drugs of Huatuo Formula by HPLC

作　　者：梁洁[1] 杨颖欣 温海成[1] 黄春燕[1] 徐晖 麦嘉妮 LIANG Jie;YANG Yingxin;WEN Haicheng;HUANG Chunyan;XU Hui;MAI Jiani(Guangxi University of Chinese Medicine,Nanning 530200,Guangxi,Chin)

机构地区：[1]广西中医药大学

出　　处：《辽宁中医杂志》2018年第6期1234-1236,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家科技部“十二五”国家科技支撑计划课题(2013BAI11B06);广西科技厅重大专项课题(1355001-4-1);广西科学研究与技术开发计划项目(桂科攻1298003-5);广西卫生厅中医药科技专项课题(GZZY13-11);壮瑶药协同创新中心(桂教科研[2013]20号);广西壮瑶药重点实验室(桂科基字[2014]32号);国家中医药管理局科研三级实验室中(壮)药化学与质量分析实验室;广西高校中药提取纯化与质量分析重点实验室;广西中医药大学“岐黄工程”高层次人才团队培育项目(2018002)

摘　　要：目的：建立同时测定壮药华陀方中绿原酸、异嗪皮啶、迷迭香酸含量的方法。方法：采用HPLC法,色谱柱为依利特Sino Chrom ODS-BP柱（4.6 mm×250 mm,5μm）,流动相为乙腈-0.2%磷酸水溶液,采用梯度洗脱程序;流速为1.0 m L·min^-1;柱温：35℃;进样量10μL;检测波长为342 nm。结果：绿原酸、异嗪皮啶、迷迭香酸分别在61.00~610.0μg·m L^-1、5.000~50.00μg·m L^-1、5.000~50.00μg·m L^-1范围内与峰面积呈良好的线性关系,重复性好,平均回收率分别为98.92%（RSD=1.143%）、99.08%（RSD=1.717%）、99.88%（RSD=1.733%）。结论：所建立的方法操作简便,具有较好的精密度和重现性,可作为壮药华陀方的质量控制方法。Objective： To establish a method for the determination of chlorogenic acid,isofraxidin and rosmarinic acid in Zhuang drugs of Huatuo Formula. Methods： The HPLC was performed on the Sino Chrom ODS-BP column（ 4. 6 mm × 250 mm,5μm） with the mobile phase of acetonitrile and 0. 2% phosphoric acid aqueous solution in gradient elution at a flow speed was 1. 0 m L·min^-1. The column temperature was 35 ℃. The injection volume was 10 μL,and the detection wavelength 342 nm. Results：The chlorogenic acid during the range of 61. 00-610. 0 μg·m L^-1 showed a good linear relation with the peak area（ r = 0. 9999）and the average recovery rate was 98. 92%,with RSD of 1. 143%（ n = 6）. The isofraxidin during the range of 5. 000-50. 00 μg·m L^-1 showed a good linear relation with the peak area（ r = 0. 9998） and the average recovery rate was 99. 08%,with RSD of1. 717%（ n = 6）. The rosmarinic acid during the range of 5. 000-50. 00 μg·m L^-1 showed a good linear relation with the peak area（ r = 0. 9998） and the average recovery rate was 99. 88%,with RSD of 1. 733%（ n = 6）. Conclusion： The method is simple and fixable. It can be used for the quantity control of Zhuang drugs of Huatuo Formula.

关 键 词：华陀方 绿原酸 异嗪皮啶 迷迭香酸 高效液相色谱法 含量 

分 类 号：R927.2[医药卫生—药学]