血清2型鸭疫里默氏杆菌铁载体受体蛋白突变体的构建及生物学特性研究  

THE MUTANT CONSTRUCTION OF THE SIDEROPHORE RECEPTOR PROTEIN AND BIOLOGICAL CHARACTERISTICS STUDY IN RIEMERELLA ANATIPESTIFER SEROTYPE 2

作　　者：董嘉文 孙敏华 李林林 马海彬 龚凤平 罗梦萍 王贵平 袁建丰 DONG Jia-wen;SUN Min-hua;LI Lin-lin;MA Hai-bin;GONG Feng-ping;LUO Meng-ping;WANG Gui-ping;YUAN Jian-feng(Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou510640, China;Guangdong Haid Institute of Animal Husbandry ＆ Veterinary, Guangzhou511400, China;Guangdong Open Labortatory of Veterinary Public Health, Guanzhou 510640, China;Key Laboratory of Livestock Disease Prevention of Guangdong Province, Guanzhou510640, China;Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture, Guanzhou510640, China)

机构地区：[1]广东省农业科学院动物卫生研究所,广州510640 [2]广东省兽医公共卫生公共实验室,广州510640 [3]广东省畜禽疫病防治研究重点实验室,广州510640 [4]农业部兽用药物与诊断技术广东科学观测实验站,广州510640 [5]广东海大畜牧兽医研究院有限公司,广州511400

出　　处：《中国动物传染病学报》2018年第3期45-50,共6页Chinese Journal of Animal Infectious Diseases

基　　金：广州市科技计划项目(201510010250;201704020083);国家重点研发计划(2017YFD0500800);广东省科技计划项目(2014A020208052;2015B020230004;2015B070701015);广东省科技发展专项资金项目(2016B020234006;2017B090904029);广东省技术开发及产业化类别(2015B020203006);广州市科技发展专项资金项目重点项目专题(201804020006);广东省新型研发机构建设--初创期补贴专题:广东省现代农业产业技术体系创新团队(2017LM2150)

摘　　要：铁离子是致病菌重要的生长因子,研究表明铁离子摄取系统调控鸭疫里默氏杆菌(Riemerella anatipestifer,RA)铁离子的代谢,是鸭疫里默氏杆菌重要的致病因子之一。为了研究RA外膜ATP依赖的铁载体受体蛋白(siderophore receptor protein,SRP)在铁离子代谢中的作用,以及SRP与细菌毒力之间的关系,本研究构建了SRP基因的突变株,通过TSB培养基和限铁环境中的生长曲线比较,结果显示突变株在限铁环境中比TSB培养基生长慢约10 h,表明SRP基因是鸭疫里默氏杆菌在体内维持铁离子代谢至关重要的成份。动物试验表明突变菌株比野生型菌株晚24 h开始死亡,说明突变菌株在体内增殖并达到致死数量的速度明显要晚于野生型,从而提示SRP是鸭疫里默氏杆菌重要的毒力因子之一。Iron is an important growth factor of pathogenic bacterium, the research shows that the iron uptake system regulates Riemerella anatipestifer iron metabolism and is one of the important pathogenic factor of Riemerella anatipestifer. In this study, the relationship between Riemerella anatipestifer ATP dependent outer membrane siderophore receptor protein（SRP） and iron metabolism, bacterial virulence factor was investigated. The mutant of SRP protein was constructed and the growth curve test showed that the growth of the mutant strain was slower about 10 hours than that of wild type strain in the iron limited environment, it indicated that SRP protein was one of the essential factors of Riemerella anatipestifer infection and had a vital role in maintaining Riemerella anatipestifer iron metabolism in vivo. The animal experiment showed that the dead time of duck infected the mutant was later 24 hours than that of the wild type strain. It showed that the growth rate of mutant strain and the speed reaching lethal number was obviously later than the wild type. Thus it indicated that SRP was one of the important virulence factor in Riemerella anatipestifer.

关 键 词：鸭疫里默氏杆菌 铁离子摄取系统 铁载体受体蛋白 毒力因子 

分 类 号：S852.617[农业科学—基础兽医学]