ICG纳米探针在早期结肠癌诊断中的应用及其价值  

Applicability of ICG nanoprobes in fluorescence molecular imaging of colon cancer

作　　者：刘志超 屈亚威[2] 刘海峰[3] LIU Zhichao;QU Yawei;LIU Haifeng(Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China;Department of Gastroenterology;Department of Medical Administration, General Hospital of Chinese People＇ s Armed Police Force, Beijing 100039 ,China)

机构地区：[1]军事科学院军事医学研究院,北京100850 [2]武警总医院消化内科,北京100039 [3]武警总医院医务部,北京100039

出　　处：《武警医学》2018年第6期586-590,共5页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的评估ICG纳米探针在结肠癌荧光分子成像中的靶向性、荧光效应及其早期诊断价值。方法建立裸鼠结肠癌皮下移植瘤模型,应用人血清白蛋白(HSA)包裹的ICG纳米探针,并以Folate Rsense^(TM)680、吲哚菁绿作为对照组,经裸鼠结肠癌皮下移植瘤模型尾静脉注射探针后,进行活体荧光分子成像,观察HAS-ICG纳米探针的成像效果,量化分析肿瘤部位的荧光信号强度。结果活体荧光分子成像结果显示:探针HAS-ICG、Folate Rsense^(TM)680均在实验瘤鼠皮下肿瘤部位出现浓聚,浓聚高峰分别在注射后1 h、24 h。与对照组相比,HAS-ICG纳米探针比商业探针有较好的靶向标记性和信噪比。结论 HSA-ICG纳米探针具有很好的标记HCT116结肠肿瘤细胞的能力,可通过荧光分子成像技术诊断早期裸鼠结肠癌变。Objective To evaluate the targeting and fluorescence effect of ICG nanoprobes in fluorescent molecular imaging of colon cancer,and to explore a new method for early diagnosis of colon cancer. Methods A nude mouse model of human colon cancer xenograft was established. An ICG nano probe packaged by human serum albumin（ HSA） was used,with Folate RsenseTM680 and indocyanine green as the control group. After intravenous injection of the probe into the colon cancer xenograft model in nude mice,the in vivo fluorescence molecular imaging（ FLI） was used to observe the HAS-ICG effect of FLI nanoparticles. The fluorescence signal intensity of tumor was quantitatively analyzed. Results In vivo fluorescence molecular imaging showed that the concentration of HAS-ICG and Folate RsenseTM680 in the subcutaneous tumor sites of experimental mice reached the peak 1 h and 24 h after injection respectively. Compared with the ICG control group,the HAS-ICG nanoprobe had a better target labeling ability and signal-to-noise ratio than the commercial probe. Conclusions The subcutaneous model of nude mouse colon cancer injected with an HSA-ICG nanoparticle probe has obvious FLI imaging effect. It has good ability to mark HCT116 colonic tumor cells,and can be used to diagnose early colon cancer in nude mice by means of fluorescence molecular imaging.

关 键 词：结肠癌 ICG纳米探针 人血清白蛋白 荧光分子成像 

分 类 号：R445[医药卫生—影像医学与核医学]