超高效液相色谱法同时测定饮用水中4种挥发酚  

Determination of 4 volatile phenols in drinking water by ultra high performance liquid chromatography

作　　者：俞涛[1] 张瑞云[1] 蒋雪凤[1] 周燕[1] YU Tao;ZHANG Rui -yun;JIANG Xue -feng;ZHOU Yan(Jianggan District Center for Disease Control and Prevention, Hangzhou, Zhejiang 310004, China)

机构地区：[1]杭州市江干区疾病预防控制中心,浙江杭州310004

出　　处：《中国卫生检验杂志》2018年第13期1554-1556,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的建立同时测定饮用水中苯酚、间甲酚、3,4-二甲酚、2,5-二甲酚的超高效液相色谱方法。方法样品直接过滤测定。采用Acquity UPLC BEH C_(18)(2.1 mm×100 mm,1.7μm)色谱柱分离,荧光检测器检测。甲醇-水(50∶50,V/V)为流动相,流速为0.20 ml/min,荧光检测器激发波长为268 nm,发射波长为310 nm。外标法定量。结果水中4种挥发酚在6 mim内完成检测,在1.0μg/L^20.0μg/L内线性关系良好(r≥0.999 7),方法的检出限为0.04μg/L^0.29μg/L,回收率为86.0%~102.6%,相对标准偏差(RSD)为0.86%~5.88%。结论该方法操作简单、快速、灵敏,可用于饮用水中挥发酚的检测。Objective To establish a method for the simultaneous determination of phenol,m-cresol,3,4-dimethylphenol,2,5-dimethylphenol in drinking water by ultra high performance liquid chromatography（ UPLC）. Methods The sample was conducted for direct filtration and determination. The analysis was performed on an Acquity UPLC BEH C（18） column（ 2. 1 mm ×100 mm,1. 7 μm） with fluorescent detection. The mobile phase was methanol-water（ 50 ∶50,V/V） and the flow rate was0. 2 ml/min. The fluorescence excitation wavelength was 268 nm,and the emission wavelength was 310 nm. External standard method was used for quantification. Results Four volatile phenols in water were detected in 6 mim. The method showed good linearity in the range of 1. 0 μg/L-20. 0 μg/L（ r≥0. 999 7）. The detection limits of the method were within 0. 04 μg/L-0. 29 μg/L,the recovery rates were within 86. 0%-102. 6%,and the relative standard deviations（ RSDs） were within 0. 86%-5. 88%. Conclusion The method is simple,fast and sensitive,and can be used for the detection of volatile phenol in drinking water.

关 键 词：超高效液相色谱 挥发酚 生活饮用水 

分 类 号：O657.72[理学—分析化学]