淫羊藿苷促进体外培养成骨细胞增殖动态观察  

Dynamic Observation of Icariin on Promoting Proliferation on the Cultured Osteoblasts in Vitro

作　　者：谢利娜[1] 刘艳辉[1] 景向东[1] XIE Lina;LIU Yanhui;JING Xiangdong(The First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong, China)

机构地区：[1]广州中医药大学第一附属医院

出　　处：《辽宁中医药大学学报》2018年第7期54-57,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：广州中医药大学青年人才培优项目;广东省医学科研基金项目(B2013165)

摘　　要：目的：动态观察淫羊藿苷（ICA）对体外培养成骨细胞的影响并探讨可能机制。方法：采用h FOB1.19人成骨细胞株体外构建人成骨细胞系,用一定浓度淫羊藿苷干预0、24、48、72 h后,通过3-（4,5-二甲基噻唑-2）-2,5-二苯基四氮唑溴盐（MTT）法观察成骨细胞增殖率,Western blotting检测OPG和BMP-2蛋白表达,实时荧光定量RT-PCR检测成骨细胞中BMP-2、OPG、ALP m RNA的表达。结果：MTT法检测表明48 h后终浓度为30μg/m L的ICA明显促进成骨细胞的增殖,与空白组相比有显著性差异（P〈0.01）;Western blotting检测能增强BMP-2、OPG表达;PCR结果显示,随时间延长,ICA明显上调BMP-2、OPG、ALP m RNA的表达,相对表达水平是加入前的3.1-7.5倍。结论：淫羊藿苷促进体外培养成骨细胞增殖,可能通过促进BMP-2、OPG蛋白表达和上调BMP-2、OPG、ALP m RNA的表达介导完成,为治疗各类骨疾病提供良好方向。Objective：To observe the effects of icariin（ICA）on the cultured osteoblasts in vitro and explore the possible mechanisms. Methods：Human osteoblasts culture system was build in vitro by h FOB 1.19 osteoblast cell lines,after intervened with a certain concentration of icariin（ICA）0,24,48,72 h,3-（4,5-dimethyl thiazol-2）-2,5-diphenyltetrazolium bromide（MTT）test was used to observe the proliferation rate of osteoblasts. The protein expression of OPG and BMP-2 were detected by Western blotting. The expression of bone morphogenetic protein 2（BMP-2）,OPG and ALP m RNA was detected by Real-time fluorescence quantitative RT-PCR. Results：The MTT assay showed that ICA with a concentration of 30 μg/m L after 48 hours was significantly promoting the proliferation of osteoblasts,there was a significant difference compared with the blank group（P〈0.01）. And,Western blotting detected that icariin can enhance the expression of BMP-2 and OPG. PCR results showed that ICA significantly increased the expression of BMP-2,OPG,ALP m RNA,and the relative expression level was 3.1-7.5 times before joining over time. Conclusion：Icariine has a direct stimulatory effect on the proliferation of cultured human osteoblast cells in vitro,which may be mediated by increasing expression of BMP-2,OPG protein and up-promotion of BMP-2,OPG and ALP m RNA expression,providing a good candidate for bone disease treatment.

关 键 词：淫羊藿苷 成骨细胞 增殖 表达 

分 类 号：R285.5[医药卫生—中药学]