产纤维素酶菌株的筛选及离子束诱变  

Screening Cellulase-producing Strains and Ion Beam Mutagenesis on Them

作　　者：刘婉 赵珊珊 焦浈[1] 杨逢源 王雁萍[1] LIU Wan;ZHAO Shan-shan;JIAO Zhen;YANG Feng-yuan;WANG Yan-ping(College of Physics and Engineering, Zhengzhou University, Zhengzhou 45000)

机构地区：[1]郑州大学物理工程学院,郑州450000

出　　处：《生物技术通报》2018年第6期149-154,共6页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31772672);河南省重点科技攻关项目(152102310064;152102110045);河南省基础与前沿技术研究项目(162300410131)

摘　　要：从含腐败秸秆的土壤中分离出刚果红水解圈与菌落比值(D/H)较大的7株纤维素酶产生菌,其中菌株S-1分解羧甲基纤维素钠的酶活(CMC酶活)最高,经鉴定为解淀粉芽孢杆菌。以之为出发菌株进行离子束诱变,采用酶标仪高通量酶活测定法筛选出CMC酶活较高的突变菌株进行传代培养,测定其传5代后菌株的CMC酶活,得到15株CMC酶活较高的突变菌株,其中突变菌株308的菌落形态变化较大,其CMC酶活最高,接种24 h后比出发菌S-1提高了84.4%,且突变菌株308的CMC酶活的提高具有遗传稳定性。表明离子束诱变对于提高菌株产纤维素酶能力具有潜在的应用价值。Seven cellulase-producing strains having large Congo red hydrolysis halo（D）and colony D/H（H is colony diameter of a strain）were isolated from soil samples containing rotten straws. Strain S-1 presented the highest CMCase activity of decomposing carboxymethylcellulose sodium,and was identified as Bacillus amyloliquefaciens. Ion beam mutagenesis was performed on S-1,and mutant strains with increased CMCase activity were screened out by high-throughput enzyme activity assay. Total 15 mutant strains with high CMCase activities after culturing for 5 generations were obtained. Among these strains,mutant strain 308,which showed totally different colony morphology,performed the highest CMCase activity. Its CMCase activity increased 84.4% after culturing for 24 h,compared to the CMCase activity of S-1;moreover,the increased CMCase activity was genetically stabile,indicating that ion beam mutagenesis has potential application value for improving the cellulase production capability of a strain.

关 键 词：解淀粉芽孢杆菌 离子束 诱变选育 纤维素酶活 

分 类 号：Q939.9[生物学—微生物学]