噪声与超氧化物歧化酶基因多态性交互作用对噪声性听力损失易感性的影响  

Combined effects of noise exposure and SOD gene polymorphism on susceptibility of occupational noise-induced hearing loss

作　　者：李旭东[1] 瞿红鹰 刘移民[2] 郑倩玲[1] 温贤忠[1] 胡世杰[1] 陈琳[2] LI Xudong;Qu Hongying;LIU Yimin;ZHENG Qianling;WEN Xianzhong;HU Shijie;CHEN Lin(Guangdong Province Hospital for Occupational Disease Prevention and Treatmen;Guangdong Provincial Key Laboratory of Occupational Diseases Prevention and Treatment, Guangzhou, Guangdong 510300, Chin)

机构地区：[1]广东省职业病防治院广东省职业病防治重点实验室,广东广州510300 [2]广州市职业病防治院,510620

出　　处：《中国职业医学》2018年第3期290-296,共7页China Occupational Medicine

基　　金：国家自然科学基金(81202179);广东省职业病防治重点实验室(2017B030314152);广东省科技计划项目(2011B050700001;2013B021800176);广东省医学科研基金(A2015150;C2016014);广州市医药卫生科技项目(20151A010076)

摘　　要：目的探讨噪声与超氧化物歧化酶(SOD)基因多态性的交互作用与中国汉族人群职业性噪声性听力损失(ONHL)易感性的关联性。方法采用单纯随机抽样法,以广州市2 400名接触40 h等效声级(L_(EX,W))为75.0~120.0 dB(A)的汉族工人为研究对象。结合其纯音测听结果和累积噪声暴露量,采用模型法确定易感组和耐受组人群(分别为201和202人),采集其周围静脉血抽提基因组DNA,采用TaqMan探针法化学荧光等位基因鉴别试验检测SOD单核苷酸多态性。采用多因素Logistic回归分析噪声接触强度与SOD交互作用。结果校正性别和重金属、粉尘、高温接触等混杂因素后,携带rs2040724 AG或GG基因型的人群交互携带rs4880(CC+CT)基因型时,罹患ONHL的风险均高于交互携带rs2040724 AA与rs4880 TT基因型者(P<0.05);携带rs10432782 GT或GG基因型的人群交互携带rs4880(CC+CT)基因型时,罹患ONHL的风险均高于交互携带rs10432782 TT与rs4880 TT基因型者(P<0.05);L_(EX,W)≥85 dB(A)人群中,交互携带rs2040724 GG与rs4880位点携带(CC+CT)基因型的人群罹患ONHL的风险高于L_(EX,W)<85 dB(A)、交互携带rs2040724 AA和rs4880 TT基因型者(P<0.01);交互携带rs10432782 GT或GG与rs4880(CC+CT)基因型的人群罹患ONHL的风险高于L_(EX,W)<85 dB(A)、交互携带rs10432782 TT与rs4880 TT基因型者(P<0.05)。结论中国汉族人群中,噪声接触强度、SOD1(rs2040724、rs10432782)、SOD2(rs4880)三者在影响ONHL易感性过程中存在交互作用。Objective To explore the association of interaction of noise exposure and superoxide dismutase（ SOD） gene polymorphism on the susceptibility of occupational noise-induced hearing loss（ ONHL） in Chinese Han population.Methods A simple random sampling method was used to select 2 400 Han workers as study subjects.These workers were exposed to 75.0-120.0 dB（ A） of normalized continuous A-weighted sound with pressure level equivalent to a 40 hworking-week（ LEX,W） in Guangzhou City.A model method was set up to define 201 sensitive workers（ sensitive group）and 202 resistant workers（ resistant group） by combining results with hearing pure tone threshold test and the cumulative noise exposure（ CNE）.The genomic DNA was extracted from peripheral blood cells.The single nucleotide polymorphisms analysis of SOD was carried out by using the TaqMan probe with chemical fluorescence allelic identification test.Binary logistic regression method was used to analyze the interaction of noise exposure and SOD.Results After adjusting confounding factors such as gender,heavy metal exposure,dust exposure and high temperature exposure,the risk for ONHL in individuals interactively carry rs2040724 AG or GG and rs4880（ CC ＋ CT） was significantly higher than that in individuals interactively carry rs2040724 AA and rs4880 TT（ P〈0.05）.The risk for ONHL in individuals interactively carry rs10432782 GT or GG and rs4880（ CC ＋ CT） was significantly higher than that in individuals interactively carry rs10432782 TT and rs4880 TT（ P〈0.05）.The risk for ONHL in individuals exposed to LEX,W≥85 dB（ A） and interactively carried with rs2040724 GG and rs4880（ CC ＋ CT） was significantly higher than that in individuals exposed to LEX,W 〈85 dB（ A） and interactively carry rs2040724 AA and rs4880 TT（ P〈0.01）.The risk for ONHL in individuals exposed to LEX,W≥85 dB（ A） and interactively carry rs10432782 GT or GG and rs4880（ CC ＋ CT） was significantly higher than that in individu

关 键 词：噪声 超氧化物歧化酶 听力损失 易感性 基因多态性 交互作用 

分 类 号：R135.8[医药卫生—劳动卫生]