针药合用对红皮病型银屑病患者外周血中T淋巴细胞亚群及TH1/TH2影响与免疫学机理分析  

Effect of Combination of Acupuncture and Medicine on T Lymphocyte Subsets and TH1/TH2 in Peripheral Blood of Patients with Erythroderma Psoriasis and Analysis of Its Immunological Mechanism

作　　者：李志锋[1] 李立红[2] 李小静[1] LI Zhifeng;LI Lihong;LI Xiao- jing(Department of Dermatology, the Affiliated Hospital of Hebei University of Engineering （Handan Hebei 056000, China;Department of Dermatology, Handan Central Hospital （ Handan Hebei 056000, China)

机构地区：[1]河北工程大学附属医院皮肤科,河北邯郸056000 [2]邯郸市中心医院皮肤科,河北邯郸056000

出　　处：《四川中医》2018年第6期173-175,共3页Journal of Sichuan of Traditional Chinese Medicine

摘　　要：目的:分析针灸对红皮病型银屑病患者外周血中T淋巴细胞亚群及TH1/TH2影响与免疫学机理。方法:选取2013年12月至2015年12月间在河北工程大学附属医院和邯郸市中心医院共同收治的红皮病型银屑病患者132例,依据随机数字表法将患者分为对照组和观察组,其中对照组(66例)口服阿维A胶囊和复方甘草酸苷片,观察组(66例)在对照组基础上进行针灸和口服凉血消银汤治疗;依据银屑病严重程度与皮损面积的评分(PASI)对患者治疗前后进行评分;使用ELISA法检测血清IL-22和IL-17含量变化情况,使用流式细胞仪检测外周血的T淋巴细胞群(CD_8^+、CD_4^+、CD_3^+、CD_4^+/CD_8^+)及Th1、Th2水平改变情况。结果:治疗后两组患者PASI评分、血清IL-22、IL-17含量均明显下降,与治疗前对比差异有统计学意义(P<0.05),且观察组效果更佳,治疗后与对照组对比差异有统计学意义(P<0.05);对照组患者仅CD_8^+和CD_4^+较治疗前有改善,差异有统计学意义(P<0.05);观察组患者治疗前CD_8^+、CD_4^+、CD_4^+/CD_8^+、Th1、Th2均有改善,差异有统计学意义(P<0.05)。结论:针灸结合凉血消银汤可明显改善红皮病型银屑病患者外周血中T淋巴细胞亚群及TH1/TH2水平,对患者临床疗效显著。Objective： Analyzing the effect of acupuncture and moxibustion on T lymphocyte subsets and TH1/TH2in periph- eral blood of patients with erythroderma psoriasis and its immunological mechanism. Methods： From December 2013to December 2015in 132patients with erythrodermic psoriasis in the Affiliated Hospital of Hebei University of Engineering and Handan Central Hospital were common cases, according to randomly divided into controlled group and observation group, the controlled group （66cases） oral A citretin capsule and Compound Glycyrrhizin Tablets, the observation group （66cases） was treated with acu- puncture and oral cooling blood and Xiaoyin Decoction on the basis of the controlled group ; The severity of psoriasis and psoriasis area the score （PASI） score of patients before and after treatment; To detect the changes of serum IL-22and IL-17content using the ELISA method, using flow cytometry to detect peripheral blood T lymphocyte subsets （CD50, CD4^＋, CD3^＋, Thl, Th2and CD4^＋/CD8^＋） level change. Results： After treatment, the two groups of patients with PASI score, serum IL-22, IL-17content decreased significantly, compared with before treatment, the difference was statistically significant （P〈0.05）, and the observation group had better effect, compared with the controlled group after treatment, the difference was statistically significant （P〈 0.05 ） ; The controlled group were only CD8^＋ and CD4^＋ compared with before treatment （P〈0. 05 ） ; Patients in the observationgroup before treatment, CD8^＋, CD4^＋, CD4^＋/CD8^＋, Thland Th2were improved significantly （P〈0. 05 ）. Conclusion： The com- bination of acupuncture combined with Liangxue Xiaoyin Decoction can obviously improve the level of T lymphocyte subgroup and TH1/TH2in peripheral blood of patients with erythroderma psoriasis, and the clinical effect is significant.

关 键 词：红皮病型银屑病 针灸 T淋巴细胞亚群 免疫学机理 

分 类 号：R758.67[医药卫生—皮肤病学与性病学] R758.63[医药卫生—临床医学]