溃结宁膏穴位敷贴对实验性溃疡性结肠炎大鼠IL-6/JAK2/STAT3信号通路的影响  

Effect of Kuijiening Ointment Acupoint Application on IL-6/JAK2/STAT3 Signaling Pathway in Ulcerative Colitis Rats with Spleen and Kidney Yang Deficiency

作　　者：毛婧 朱莹[2] 周瑾 陈凯[1] 陈程 Mao Jing;Zhu Ying;Zhou Jin(Hunan University of Chinese Medicine ,Hunan , Changsha 410208, China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南中医药大学第二附属医院,湖南长沙410005

出　　处：《中国中医急症》2018年第7期1167-1170,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：湖南省教育厅科学研究重点项目(16A164)

摘　　要：目的观察溃结宁膏穴位敷贴对实验性溃疡性结肠炎大鼠结肠组织IL-6、gp130、JAK2、STAT3的影响,并探讨其机制。方法将52只SD大鼠随机分为空白组、模型组、敷贴组、SASP组,采用复合法建立脾肾阳虚型溃疡性结肠炎大鼠模型(腺嘌呤、冰番泻叶药汁分阶段灌胃,外因干预,噁唑酮灌肠),造模成功后分组予以溃结宁膏穴位敷贴、SASP灌胃,观察各组大鼠症状、体征、结肠黏膜组织病理,Western blot检测大鼠结肠组织白细胞介素-6(IL-6)、gp130、JAK2、STAT3蛋白变化。结果与模型组相比,敷贴组和SASP组大鼠症状、体征及结肠组织学均有不同程度改善;与空白组相比,模型组、敷贴组及SASP组大鼠结肠组织IL-6、gp130、JAK2、STAT3表达显著增高(P<0.01);与模型组相比,敷贴组与SASP组IL-6、gp130、JAK2、STAT3表达水平均降低(均P<0.01)。结论溃结宁膏穴位敷贴治疗实验性溃疡性结肠炎的可能机制是抑制IL-6/JAK2/STAT3信号通路来发挥疗效。Objective： To observe the effect of Kuijiening Ointment Acupoint Application（KJNAA） on IL-6,gp130,JAK2 and STAT3 in ulcerative colitis rats with spleen and kidney Yang deficiency,and to explore its treatment mechanism on ulcerative colitis. Methods： 52 SD rats were randomly divided into the blank group,the model group,the application group and SASP group. Rat model of ulcerative colitis of spleen and kidney Yang deficiency was established with compound method（intragastric administration of Adenine and senna ice juice,external cause intervention,Oxazole enema） After successful modeling,KJNAA were applied and SASP was administered orally to observe the symptoms and signs of the rats in each group,as well as histopathology of colonic mucosa. IL-6,gp130,JAK2 and STAT3 in colonic tissue were detected by Western blot. Results： Compared with the model group,the symptoms,signs and colon histology of the application group and SASP group were improved to some extent. Compared with the blank group,the expression of IL-6,JP130,JAK2 and STAT3 in the model group,the application group and SASP group were significantly increased. Compared with the model group,the expression levels of IL-6,gp130,JAK2 and STAT3 in the application group and SASP group were all decreased.Conclusion： The possible mechanism of KJNAA on ulcerative colitis of spleen and kidney Yang deficiency is to inhibit the IL-6/JAK2/STAT3 signaling pathway.

关 键 词：溃疡性结肠炎 脾肾阳虚 大鼠 溃结宁膏 穴位贴敷 

分 类 号：R245.9[医药卫生—针灸推拿学]