木霉融合子Tpf-2的定殖及其对番茄防御酶系的影响  

The rhizosphere soil colonization of Trichoderma fusant Tpf-2 enhances defence enzyme activities against tomato damping-off

作　　者：李纪顺 陈凯 李玲[1] 赵忠娟 杨玉忠 杨合同 LI Jishun;CHEN Kai;LI Ling;ZHAO Zhongjuan;YANG Yuzhong;YANG Hetong(Ecology Institute,Qilu University of Technology（Shandong Academy of Sciences）,Shandong Provincial Key Laboratory of Applied Microbiology,Jinan 250103,China)

机构地区：[1]齐鲁工业大学(山东省科学院)山东省科学院生态研究所山东省应用微生物重点实验室,济南250103

出　　处：《植物保护》2018年第4期65-69,91,共6页Plant Protection

基　　金："十三五"国家重点研发计划(2017YFD0201102;2017YFD0201706);山东省科学院青年基金(2017QN009)

摘　　要：利用qPCR检测木霉融合子Tpf-2在番茄根际土中的定殖,并采用生理生化法测定Tpf-2对番茄叶片中相关防御酶活性的影响。结果表明,在整个番茄生育期内(0~150d),Tpf-2均可在番茄根际土中定殖,相对定殖数量随时间的延长呈下降趋势,同时接种木霉和瓜果腐霉处理组中Tpf-2相对定殖数量高于同期无病原菌处理组。不同处理组番茄叶片中的超氧化物歧化酶SOD、过氧化物酶POD及过氧化氢酶CAT的活性不同,同时接种木霉和瓜果腐霉处理组中上述3种酶活性增加显著,与只接种瓜果腐霉处理组间存在极显著性差异(P<0.01)。综上可见,木霉融合子Tpf-2的厚垣孢子定殖周期长,定殖后可诱导番茄叶片中相关防御酶活性的提高,本研究为揭示其生防机理及菌株应用提供了科学依据。The rhizosphere soil colonization dynamics of Trichoderma fusant Tpf-2 chlamydospore and its systemic resistance mechanism against tomato damping-off were studied by using quantitative PCR（qPCR）and physiological and biochemical analyses.The results showed that Trichoderma fusant Tpf-2 could colonize rhizosphere soil during the whole tomato growth period（0-150 days）;the relative colonization quantity decreased along with planting time,and the relative colonization quantity after inoculated with Pythium aphanidermatum exceeded that without inoculation with P.aphanidermatum.The activities of superoxide dismutase（SOD）,catalase（CAT）and peroxidase（POD）in tomato leaves were different among different treatments,and enhanced remarkably when inoculated with Trichoderma and P.aphanidermatum,significantly different from those when inoculated with P.aphanidermatum only（P〈0.01）.These results indicated that the colonization of Trichodermafusant Tpf-2 chlamydospore in tomato rhizosphere soils lasted for a long time,and could induce the activities of defence enzymes,which provided the scientific basis for Trichoderma fusant Tpf-2 application and further research on biocontrol mechanisms.

关 键 词：木霉融合子Tpf-2 番茄猝倒病 瓜果腐霉 定殖 防御酶活性 

分 类 号：S476[农业科学—农业昆虫与害虫防治]