2型糖尿病患者绝经后Dickkopf-1(Dkk1)蛋白表达与骨代谢关系研究  

Relationship analysis between Dkk1 protein and bone metabolism in postmenopausal with type 2 diabetes mellitus patients

作　　者：李佳佳 李军[1] 李思源[2] 胡颖 孙侃[1] 胡倩 王晓丽[1] LI Jiajia;LI Jun;LI Siyuan(Department o f Endocrinology and Metabolic,The First Affiliated Hospital,School of Medicine,Shihezi University,Shihezi 832000,Chin)

机构地区：[1]新疆石河子大学医学院第一附属医院内分泌代谢科,832000 [2]新疆石河子大学医学院组胚教研室 [3]新疆医科大学第四附属医院神志病科

出　　处：《中国糖尿病杂志》2018年第7期573-576,共4页Chinese Journal of Diabetes

基　　金：兵团科技援疆基金(2014AB049);兵团中青年科技创新领军人才专项基金(2015BC001)

摘　　要：目的 探讨T2DM患者绝经后Dickkopf-1(Dkk1)蛋白表达水平与骨代谢的关系。方法 收集新疆石河子地区绝经后T2DM女性50例(T2DM组)与糖耐量正常(NGT组)女性50名,检测临床代谢指标。ELISA测定血清Dkk1蛋白表达水平。结果 与NGT组比较,T2DM组FPG、2hPG、果糖胺(FMN)、HbA1c、血P水平升高[(5.47±0.73)vs(9.26±2.38)mmol/L,(6.37±0.62)vs(16.66±3.27)mmol/L,(239.74±36.54)vs(337.66±87.31)μmol/L,(6.23±0.92)%vs(8.70±1.76)%,(1.11±0.12)vs(1.24±0.16)mmol/L;P<0.01];骨钙素(OC)、25羟维生素D3[25(OH)D3]、骨密度[BMD(Femur)]水平降低[(30.25±7.41)vs(19.27±5.07)ng/ml,(35.37±6.83)vs(22.00±5.26)nmol/L,0.87(0.80,0.95)vs 0.80(0.61,0.90)g/cm2;P<0.01];Dkk1蛋白表达水平升高[(209.37±32.35)vs(232.97±39.31)pg/ml,P<0.05]。相关分析显示,Dkk1蛋白表达与血P呈正相关(r=0.301,P<0.05),与BMD(Femur neck)呈负相关(r=-0.349,P<0.01)。BMD(Femur)与FPG呈负相关,与OC呈正相关(r=-0.344、0.267,P<0.01)。多元线性回归分析显示,25(OH)D3是BMD(Femur)的影响因素。结论 Dkk1蛋白的高表达可能参与了T2DM患者绝经后BMD降低及血P升高,OC、25(OH)D3等骨代谢指标降低,以此为靶点,有利于防治骨质疏松(OP)。Objective To analyze the relationship between Dickkopf-1（Dkk1）protein expression and bone metabolism in postmenopausal women with type 2 diabetes mellitus（T2 DM）. Methods A total of 100 subjects were collected in Shihezi area in Xinjiang province and divided into two groups：patient with T2 DM（T2 DM group,n=50）and healthy subjects（NGT group,n=50）.Clinicalin formation was measured and recorded.The expression of serum Dkk1 protein was determined by enzyme linked immunosorbent assay（ELISA）. Results The level of FPG,2 hPG,Froctosamine（FMN）,HbA1 c and P were higher in T2 DM group than in NGT group[（5.47±0.73）vs（9.26±2.38）mmol/L,（6.37±0.62）vs（16.66±3.27）mmol/L,（239.74±36.54）vs（337.66±87.31）μmol/L,（6.23±0.92）% vs（8.70±1.76）%,（1.11±0.12）vs（1.24±0.16）mmol/L;P〈0.01].The level of Osteocalcin（OC）,25（OH）D3,BMD（Femur）were lower T2 DM group than in NGT group[（30.25±7.41）vs（19.27±5.07）ng/ml,（35.37±6.83）vs（22.00±5.26）nmol/L,0.87（0.80,0.95）vs 0.80（0.61,0.90）g/cm^2;P〈0.01].The expression of Dkk1 proteinwas higher in T2 DM group than in NGT group[（209.37±32.35）vs（232.97±39.31）pg/ml,P〈0.05].Correlation analysis showed that the expression of Dkk1 protein was positively correlated with P（r=0.301,P〈0.05）and negatively correlated with BMD（Femur neck）（r=-0.349,P〈0.01）.BMD（Femur）was negatively correlated with FPG and positively related with OC（r=-0.344,0.267,P〈0.01）.Multiple linear regression showed that 25（OH）D3 was a risk factor for BMD（Femur）. Conclusion The high expression of Dkk1 protein may be involved in the decrease of BMD,the increase of P and the decrease of OC and 25（OH）D3 in postmenopausal women with T2 DM.Dkk1 protein may be a target for the prevention and treatment of osteoporosis（OP）in postmenopausal women with T2 DM.

关 键 词：绝经后 糖尿病 2型 Dickkopf-1蛋白 骨密度 

分 类 号：R580[医药卫生—内分泌] R587.2[医药卫生—内科学]