高血压患者血清microRNA-92a水平与大动脉僵硬度的关系  

The Level of Serum MicroRNA-92a in Patients with Hypertension and Its Relationship with Arterial Stiffness

作　　者：叶玉兰 尚粉青[3] 李齐斐[4] 王亭忠 马爱群[1] YE Yu-lan;SHANG Fen-qing;LI Qi-fei;WANG Ting-zhong;MA Ai-qun(：Department of Cardiovascular Medicine, the First Affiliated Hospital of Xi ＇an Jiaotong University, Xi ＇an, Shaanxi, 710061, China;：Cardiovascular Department of Xi ＇an Gaoxin Hospital Xi ＇an, Shaanxi, 710075, China;, Cardiovascular Department of Xi ＇an No. 1 Hospital Xi ＇an, Shaanxi, 710002, China;Physical Examination Department of Xi ＇an Gaoxin Hospital, Xi ＇an, Shaanxi, 710075, China.)

机构地区：[1]西安交通大学附属第一医院心血管内科,西安市7100061 [2]西安高新医院心血管内科,西安市710075 [3]西安市第一医院心血管内科,西安市710002 [4]西安高新医院体检科,西安市710075

出　　处：《中国分子心脏病学杂志》2018年第3期2457-2461,共5页Molecular Cardiology of China

摘　　要：目的观察高血压患者血清micro RNA-92a(mi R-92a)水平及其与大动脉僵硬度的关系。方法入选113例高血压患者与54例健康体检者,采用实时荧光定量PCR(quantitative real-time PCR)技术检测血清中mi R-92a水平,采用酶联免疫吸附测定(ELISA)法测定血清中血清内皮素-1(endothelin-1,ET-1)水平,采用硝酸还原酶法测定血清中一氧化氮(nitric oxide,NO)含量,采用动脉硬化自动测量仪测量肱踝动脉脉搏波传导速度(brachial-ankle pulse wave velocity,ba PWV)。18-20周的SHR大鼠(WKY做对照组),适应环境1周后测定鼠尾动脉血压,采用Western Blot、Real time–PCR分析及测定主动脉内皮细胞mi R-92a、ET-1水平及e NOS含量。取大鼠主动脉组织行石蜡包埋、切片、HE染色及Masson染色,观察动脉中层结构改变及胶原纤维含量的变化。结果高血压患者血清mi R-92a、ET-1水平显著升高,血清NO含量明显降低,ba PWV明显增加(P值均<0.001)。采用Spearman相关性分析发现mi R-92a与血清ET-1水平(相关系数0.74)及ba PWV(相关系数0.68)呈显著正相关,而与血清NO含量呈显著负相关(相关系数0.77)。SHR大鼠血压明显升高,与WKY相比有显著性差异(P值均<0.0001)。Real time-PCR结果提示SHR大鼠主动脉内皮细胞mi R-92a及ET-1表达明显增加;e NOS的表达明显减少(P值均<0.05);Western Blot分析SHR大鼠主动脉组织中ET-1表达增加,而phospho-e NOS(S1177)表达减少(P值均<0.05)。主动脉HE染色及Masson染色可见SHR大鼠动脉平滑肌细胞增殖,中层增厚,胶原纤维含量明显增加。结论高血压患者血清mi R-92a水平升高,影响ET-1及e NOS的表达,导致动脉中层纤维含量大量增加,大动脉僵硬度显著增加。Objective To observe the level of serum micro RNA-92 a（mi R-92 a） in patients with hypertension and its relationship with arterial stiffness. Methods 113 patients with hypertension and 54 healthy subjects were included. The serum levels of mi R-92 a, endothelin-1（ET-1） and nitric oxide（NO） were detected using real-time fluorescence quantitative PCR, ELISA assay and nitrate reductase method, respectively. The brachial ankle pulse wave velocity（ba PWV） was also measured. 18-20 weeks of SHR rats（WKY served as control group） were included, the blood pressure of rat tail artery was measured after 1 week of adaptation to the environment. Western Blot, Real time-PCR analysis and determination of mi R-92 a and ET-1 levels, and e NOS contents in aortic endothelial cells were performed. Paraffin-embedded, aliquoted, HE stained, and Masson stained rat aortic tissues were used to observe the change of the middle artery structure and the changes of collagen fiber content. Results Serum mi R-92 a and ET-1 levels and ba PWV were significantly elevated in hypertensive patients, while serum NO levels were significantly decreased（P〈0.001）. Spearman＇s correlation analysis showed, that mi R-92 a was positively correlated with serum ET-1（R=0.74） and the ba PWV（R=0.68） but negatively correlated with the serum NO concentration（R=-0.77）. The blood pressure of SHR rats was significantly higher than that of WKY（P〈0.0001）. Real time-PCR results showed that the expression of mi R-92 a and ET-1 in aortic endothelial cells of SHR rats significantly increased; the expression of e NOS was significantly decreased; the expression of ET-1 in the aorta of SHR rats was increased by Western Blot analysis, while phospho-e NOS（S1177） was decreased（P〈0.05）. Aortic HE staining and Masson staining showed that SHR rat arterial smooth muscle cell proliferation, thickening of the middle layer, collagen fiber content increased significantly. Conclusions The level of serum mi R-92 a is elevated in hyperte

关 键 词：高血压 microRNA-92a 大动脉僵硬度 肱踝动脉脉搏波传导速度 

分 类 号：R544.1[医药卫生—心血管疾病]