高糖对心肌细胞t小管结构和JP-2表达的影响  

Effects of High Glucose on the Structure of T-tubule and Expression of JP-2 in Cardiomyocytes

作　　者：周丹丹[1] 王毅晖[1,2] 赵理栋 洪江 ZHOU Dan-dan;WANG Yi-hui;ZHAO Li-dong;HONG Jiang(Shanghai General Hospital, Shanghai Jiao Tong University Shanghai 200080, China;Division of Cardiovascular Medicine University, of lowa Carver College of Medicine, Iowa City 52242, USA.)

机构地区：[1]上海交通大学附属第一人民医院,上海市200080 [2]美国爱荷华大学医学院

出　　处：《中国分子心脏病学杂志》2018年第3期2509-2512,共4页Molecular Cardiology of China

基　　金：国家自然科学基金(81570293);松江区科委科技攻关项目(2017031);上海市卫计委重要薄弱学科建设项目(2016ZB0205)

摘　　要：目的研究高糖对小鼠心肌细胞横管(t小管)结构和横管结构蛋白亲联蛋白2(junctophilin-2,JP-2)表达的影响,探讨高糖致心脏损害的可能机制。方法获取小鼠心室肌细胞分离培养,并分成两组分别给予高糖和低糖处理。激光共聚焦显微镜成像技术记录两组细胞钙火花(Ca^(2+)spark)变化,测定自发性钙火花频率(Ca^(2+)spark frequency)变化;观察心肌细胞t小管结构变化;免疫印迹法检测JP-2蛋白表达;免疫荧光检测JP-2定位。结果高糖可以明显增加心室肌细胞的自发性钙火花频率,并且高糖对t小管结构的破坏显著,降低t小管上JP-2蛋白的表达。结论高糖通过降低JP-2蛋白的表达进而损害心肌细胞t小管,使其结构发生紊乱,从而增加心室肌细胞自发性钙火花频率。Objective To identify the effect of high glucose on the structure of transverse tubule（t-tubule） and expression of junctophilin-2（JP-2） in mouse cardiomyocytes and to explore the potential mechanism of high glucose-induced cardiac damage. Methods Mouse ventricular myocytes were collected and cultured and divided into two groups with treatment of high glucose and low glucose, respectively. Laser confocal microscopy imaging was used to record the calcium sparks and the structure of t-tubule in both groups. The expression of JP-2 in ventricular myocytes was detected by western blotting and immunofluorescence. Results High glucose significantly increased the frequency of spontaneous calcium sparks and the destruction of t-tubules in ventricular myocytes. In addition, high glucose decreased the expression of JP-2 in t-tubules. Conclusions High glucose can damage the structure of t-tubule by decreasing the expression of JP-2, thereby increasing the frequency of spontaneous calcium sparks in ventricular myocytes.

关 键 词：高糖 心肌损伤 钙火花 t小管 亲联蛋白2 

分 类 号：R542.2[医药卫生—心血管疾病] R587.1[医药卫生—内科学]