不同来源白念珠菌感染小鼠前后胞外水解酶活力差异  

Comparison of the activity of extracellular hydrolytic enzyme produced by Candida albicans in vitro and in mice

作　　者：夏茂宁 邱文 牛雪可 王梅竹 赵亮[1,2] 张金娟[3] 田敏[1,2] 廖万清 康颖倩[1,2] XIA Mao-ning1,2, QIU Wen1,2, NIU Xue-ke1,2, WANG Mei-zhu1,2, ZHAO Liang1,2, ZHANG Jin-juan3, TIAN Min1,2, LIAO Wan-qing4, KANG Ying-qian1,2(1. Department of Microbiology, Guizhou Medical University, Guiyang 550025, China; 2. Key Laboratory of Pathogenic Biology of Guizhou High Education Institution ＆ Key Laboratory of Environmental Pollution Monitoring and Disease Control of Ministry of Education, Guiyang 550025, China; 3. Laboratory of Functions, Guizhou Medical University, Guiyang 550025, China; 4. Department of Dermatology and Venereal Disease, Changzheng Hospital, Navy Medical University, Shanghai 200003, China)

机构地区：[1]贵州医科大学微生物学教研室,贵阳550025 [2]贵州省普通高等学校病原生物学特色重点实验室暨环境污染与疾病监控省部共建教育部重点实验室,贵阳550025 [3]贵州医科大学机能实验室,贵阳550025 [4]海军军医大学附属长征医院皮肤科,上海200003

出　　处：《中国人兽共患病学报》2018年第7期613-618,634,共7页Chinese Journal of Zoonoses

基　　金：贵州省卫生计生委科学技术基金项目(gzwjkj 2016-1-049);贵阳市人民政府-贵州医科大学科学技术联合基金"贵阳市科技局创新团队"项目(GY2015-18);贵州省优秀青年人才科技培养项目(黔科合平台人才[2017]5639);贵阳市人民政府-贵州医科大学科学技术联合基金项目(GY2016-20)联合资助~~

摘　　要：目的了解白念珠菌进入小鼠宿主前后其分泌胞外水解酶能力是否改变。方法挑选分泌型天冬氨酸蛋白酶、磷脂酶、脂肪酶活力无差异的艾滋病、外阴阴道念珠病病患来源和健康人来源白念珠菌,尾静脉注射感染小鼠,分离发病死亡小鼠肾脏菌株,比较感染小鼠前后菌株胞外水解酶活力差异。结果不同来源菌株感染的小鼠28d内死亡速率差异显著(χ2=82.5,P<0.05),从高到低依次为艾滋病、外阴阴道念珠病、健康人来源组;各来源菌株感染小鼠后肾脏分离株分泌型天冬氨酸蛋白酶表达水平皆高于感染小鼠前(F=7.89,P<0.05),不同来源区别显著(F=4.96,P<0.05),从高到低依次为艾滋病、外阴阴道念珠病、健康人来源;感染小鼠前均未曾测得磷脂酶活力的各来源菌株中,在感染小鼠后仅艾滋病、外阴阴道念珠病来源组小鼠肾脏分离株可测得磷脂酶活力,但艾滋病、外阴阴道念珠病来源两组间感染小鼠后肾脏分离株磷脂酶活力无显著差异(F=2.54,P>0.05);各来源白念珠菌感染小鼠前后均未测得脂肪酶活力。结论在宿主体内白念珠菌表达天冬氨酸蛋白酶、磷脂酶能力相较于体外有增强;在不同宿主环境状态下,白念珠菌分泌型天冬氨酸蛋白酶和磷脂酶的潜在表达能力有差异,即宿主免疫状态越弱时,白念珠菌分泌型天冬氨酸蛋白酶、磷脂酶潜在表达能力越强。Extracellular hydrolytic enzymes have been proved as part of the virulence factors of Candida albicans in vitro. However, how those enzymes work is still unknown when the C. albicans cells＇ growth environment have been changed from the vitro to the host. Therefore, we analyzed the secreted aspartyl proteinase （SAP）, lipase （Lip） and phospholipase （PL） from the C. albicans cells isolated from AIDS, vulvovaginal candidiasis（VVC） patients and normal people. During our study, female KM mice were treated with above C. albicans cells from various sources by tail vein injection, and the C. albicans cells were collected from the mice kidneys when they died. And the activities of extracellular hydrolases produced by the selected strains of C. albicans were compared between in vitro and in vivo of mice respectively. The death rate showed significant difference in the mice treated with C. albicans cells from various sources within 28 d, and the death rate caused by C. albicans isolates from AIDS patients was higher than those form VVC and normal persons. Especially the expression levels of SAP of the strains isolated from mice were higher than in vitro, obvious difference was observed （F=7.89,P〈0.05）, and the differences were more significant in C. albicans isolates from AIDS patients than those from VVC and normal persons. On the other hand, the PL activity was tested as negative when the C. albicans cells cultured on the artificial media, but this activity changed into positive when C. albicans cells from AIDS and VVC sources grew inside the mice’s bodies, which showed no significant difference between these two groups（F=2.54,P〉0.05）. In addition, the activities of Lip were not detected in all the strains. In conclusion, the secretion amount of SAP and PL of C. albicans in vivo in mice is enhanced than that in vitro. Under different host environmental conditions, the potential expression abilities of SAP and PL of C. albicans are different, that means the weaker the host immune status, the

关 键 词：白念珠菌 分泌型天冬氨酸蛋白酶 磷脂酶 脂肪酶 小鼠模型 

分 类 号：R378.99[医药卫生—病原生物学]