基于转录组学的不同色系蝴蝶兰花色苷差异积累分析  

Study on the Differential Accumulation of Anthocyanin in Different-colored Phalaenopsis Based on Transcriptomics

作　　者：张加强[1] 史小华[1] 刘慧春[1] 马广莹[1] 邹清成[1] 朱开元[1] 周江华[1] 毛军铭[1] Zhang Jiaqiang;Shi Xiaohua;Liu Huichun;Ma Guangying;Zou Qingcheng;Zhu Kaiyuan;Zhou Jianghua;Mao Junrning(Flower Research and Development Center, Zhejiang Academy of Agricultural Sciences, Hangzhou, 311202)

机构地区：[1]浙江省农业科学院花卉研究开发中心,杭州311202

出　　处：《分子植物育种》2018年第14期4530-4542,共13页Molecular Plant Breeding

基　　金：浙江省农科院青年科技人才培养项目(2015R25R08E03;2017R25R08E02);2016年度浙江省农科院中青年科技人员国内外进修培养项目;浙江省公益技术应用研究计划项目(2017C32039)共同资助

摘　　要：蝴蝶兰花色丰富且极具变化,是研究花着色机制的理想材料。本研究利用RNA Sequencing(RNA-Seq)技术比较三种不同色系蝴蝶兰花器官转录组,鉴定蝴蝶兰花色苷合成的相关基因,从转录组水平揭示蝴蝶兰花色苷生物合成的分子调控机制。选取白花蝴蝶兰(PAPW)、红花蝴蝶兰(PAPR)和黄花蝴蝶兰(PAPY)为试验材料,对其花苞及盛开花朵分别釆样,分别提取三个样品的总RNA,利用Illumina Hiseq 2 000进行测序分析,过滤处理后分别得到总Clean reads片段为60 031 912、57 685 822和55 765 402个,GC含量分别为47.78%、47.36%和49.48%,平均长度约为575 bp,N50长度为940 bp,对获得的All-unigenes进行功能注释,注释到Swiss-Prot、Nt、KO、Nr、GO和KOG数据库的Unigenes分别是22 321、19 199、8 671、28 952、20 152和10 380个;PAPR和PAPW相比差异基因共有642个,上调基因有354个,下调基因有288个;PAPY和PAPW相比差异基因共有2 459个,上调基因有132个,下调基因2 327个;KEGG代谢分析表明,2个对比组中的差异基因富集在不同代谢通路中。PAPR和PAPW差异基因主要富集在类黄酮生物合成,苯丙氨酸代谢和钙信号通路等代谢通路,其中类黄酮生物合成中差异基因为8个(7个上调,1个下调)。PAPY和PAPW差异表达基因主要富集在DNA复制,烷、哌啶和吡啶生物碱和细胞凋亡等代谢通路,其中,参与到淀粉与蔗糖代谢中的差异基因最多,有34条(1个上调,33个下调)。这些信息为蝴蝶兰不同花色苷形成相关关键基因的研究提供了重要依据。Phalaenopsis is rich in col or and varies greatly,which is an ideal material for studying the coloring mechanism of flowers.In this study,we used RNA sequencing（RNA-Seq） technology to compare the flower organs of three different-colored phalaenopsis,identify the genes related to anthocyanin biosynthesis in phalaenopsis and reveal the molecular regulation mechanism of anthocyanin biosynthesis in phalaenopsis from transcriptome level.White flower phalaenopsis（PAPW）,red flower phalaenopsis（PAPR） and yellow flower phalaenopsis（PAPY） were selected as test materials,and their buds and flowers were sampled.The total RNA of three samples were extractedand Illumina Hiseq 2 000 was used to sequence.After filtration treatment,the total Clean reads fragments were 60031 912,57 685 822 and 55 765 402,respectively.The GC contents were 47.78%,47.36% and 49.48%,respectively,with the average length of 575 bp and N50 length of 940 bp.Functional annotation was carried out on the acquired All-unigenes,the results of which showed that the number of Unigenes of Swiss-Prot,Nt,KO,Nr,GO and KOG database were 22 321,19 199,8 671,28 952,20 152 and 10 380,respectively.A total of 642 differentially expressed genes（DEGs） were screened out from PAPR vs PAPW,in which 354 DEGs were up-regulated and 288 DEGs were down-regulated.A total of 2 459 DEGs were screened out from PAPY vs PAPW,including132 up-regulated and 2 327 down-regulated.The KEGG metabolism analysis indicated that these DEGs from two contrast groups gathered in different metabolic pathways.The DEGs from PAPR vs PAPW mainly gathered in metabolic pathways such as flavonoid biosynthesis,phenylalanine metabolism and calcium signaling pathways,and there were 8 DEGs in flavonoid biosynthesis pathways（7 up-regulated and 1 down-regulated genes）.While the DEGs from PAPY vs PAPW mostly gathered in metabolic pathways such as DNA replication,alkane,piperidine and pyridine alkaloids and cell apoptosis.The DEGs involved in starch and sucrose metabolism were the most,with th

关 键 词：蝴蝶兰 不同色系 花色苷 转录组测序 差异表达基因 

分 类 号：S682.31[农业科学—观赏园艺]