不同鸡种BF2基因序列比较分析  

Comparative Analysis of BF2 Gene Sequences in Different Chicken Breeds

作　　者：郑圣晗 陈颖[1] 孔令琳[1] 吴允[1] 郭其新[1] 陈国宏[1] 常国斌[1] ZHENG Shenghan;CHEN Ying;KONG Linglin;WU Yun;GUO Qixin;CHEN Guohong;CHANG Guobin(College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Chin)

机构地区：[1]扬州大学动物科学与技术学院,扬州225009

出　　处：《吉林农业大学学报》2018年第3期345-351,共7页Journal of Jilin Agricultural University

基　　金：国家科技支撑计划项目(2015BAD03B03);江苏高校优势学科建设工程资助项目

摘　　要：利用目标区域捕获测序技术对BF2(Major histocompatibility complex class I antigen BF2)基因进行序列测定,找出不同鸡种BF2基因序列上的差异。以NCBI网站上Gallus_gallus-5.0的BF2基因序列为参照标准。利用MEGA6.06软件对25个鸡种的基因序列进行比对分析,统计SNPs位点和Indel位点,并构建系统进化树进行分析。结果表明:在BF2第2、3外显子上分别存在22和31个有义突变,占BF2基因全部有义突变的78%,表明BF2基因的第2、3外显子起重要作用且拥有丰富的多态性。第5、6、7外显子属于保守区域。25个鸡种大致可被分为3大类:鹿苑鸡、如皋黄鸡、溧阳鸡和太湖鸡等聚为一类;罗斯鸡和隐性白羽肉鸡等聚为一类;雪山鸡和广西黄鸡等聚为一类。表明中国地方鸡品种间的同源性较小,BF2基因在不同鸡种上具有丰富的遗传多样性,在第2外显子上C/T突变和第3外显子上A/C突变可作为BF2基因有关抗病的候选SNPs位点。In this study,the differences of BF2（ Major histocompatibility complex class I antigen BF2） gene in different chicken breeds were investigated by target region capture sequencing technology,which will provide basic materials for breeding disease-resistance chicken breeds. The BF2 gene sequence of Gallusgallus-5. 0 on the NCBI website was used as the reference. The gene sequences of 25 chicken breeds were compared and analyzed by MEGA6. 06 software. SNPs and Indel loci were counted and phylogenetic trees were constructed for analysis. The results showed that 22 and 31 missense mutations in exon 2 and 3 accounted for 78% of missense mutations of BF2 gene,which indicated that exons 2 and 3 of BF2 gene played an important role and had rich polymorphism.The exons 5,6 and 7 belong to the conserved region. The 25 chicken species could be roughly divided into three categories,Luyuan chicken,Rugao chicken,Liyang chicken and Taihu chicken; Ross chicken and recessive white chicken; Xueshan chicken and Guangxi yellow chicken were clustered into one group,respectively,which showed that the homology among Chinese local chicken breeds was low and that BF2 gene had abundant genetic diversity in different chicken breeds. C/T mutations in exon 2 and A/C mutations in exon 3 of BF2 gene could be candidate SNPs for disease resistance research in Chicken.

关 键 词：鸡 BF2基因 SNPs位点 Indel位点 进化树 

分 类 号：S831.2[农业科学—畜牧学]