大豆NIP类水孔蛋白基因的鉴定及表达特性分析  

Genome-wide identification and expression analysis of soybean NIPs

作　　者：吴伟 冯志娟[2] 徐盛春[2] 刘娜 张古文[2] 胡齐赞[2] 龚亚明[2] WU Wei;FENG Zhijuan;XU Shengchun;LIU Na;ZHANG Guwen;HU Qizan;GONG Yaming(Zhejiang Provincial Seed Management Station,Hangzhou 310020,China;Institute of Vegetables,Zhejiang Academy of Agricultural Seiences,Hangzhou 310021,China)

机构地区：[1]浙江省种子管理总站,浙江杭州310020 [2]浙江省农业科学院蔬菜研究所,浙江杭州310021

出　　处：《浙江农业学报》2018年第7期1101-1109,共9页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金(31601767;31572138);浙江省农业科学院青年人才培养项目(2016R23R08E06);浙江省蔬菜新品种选育重大科技专项(2016C02051)

摘　　要：NIPs(nodulin 26-like intrinsic proteins)是类根瘤菌26膜内在蛋白,属于水孔蛋白的一个亚类,在植物逆境胁迫应答过程中发挥重要作用。利用大豆基因组数据库,在全基因组水平共鉴定得到13个GmNIPs。染色体定位分析显示,这些GmNIPs基因分布在大豆11条染色体上。理化性质分析显示,GmNIPs蛋白氨基酸数目为261~296,蛋白分子量为27~32 ku,蛋白等电点为6~10。蛋白多序列比对分析发现,所有GmNIPs均含有水通道蛋白的典型结构,6个保守的跨膜螺旋(TM1-TM6)和2个保守的氨基酸元件NPA盒(Asp-ProAla box)。进化树分析发现,大豆GmNIPs与拟南芥和水稻NIPs分类完全一致。基因结构分析显示,所有GmNIPs基因均由5个外显子和4个内含子组成。启动子分析表明,多数GmNIPs基因的上游启动子区含有逆境和激素应答元件。组织表达分析发现,5个GmNIPs的基因表达量相对较高。进一步,利用荧光定量PCR对Gm NIP1;5的干旱胁迫表达特性进行了分析。Nodulin 26-like intrinsic protein （NIPs） was one subclass of aquaporins which played vital roles in abiotic stress responses. Based on soybean genome database, a total of 13 GmNIP genes were identified on the genome level. Chromosome location analysis showed that these GmNIPs located on 11 chromosomes. Physical and chemical properties analyses revealed that the amino acid number of GmNIP proteins ranged from 261 to 296, with molecular masses of 27 to 32 ku and isoelectronic points of 6 to 10. Protein multiple alignments showed that all GmNIPs contained the typical regions of aquaporins： six putative trans-membrane domains （TM1 to TM6） and two NPA （Asp-Pro-Ala box） domains. Phylogenetic analysis showed that the classification of GmNIPs from soybean was consistent with Arabidopsis and rice NIPs. Gene structure analysis revealed that all GmNIPs were consisted of five exons and four introns. Promoter region analysis indicated that the upstream promoter region of most GmNIPs had stress and hormone responsive elements. Tissue expression analysis suggested that five GmNIPs had relatively high transcripts. Furthermore, the expression profile of GmNIP 1;5 in response to drought stress was analyzed by the real-time PCR.

关 键 词：大豆 NIP 水孔蛋白 全基因组鉴定 组织表达 干旱应答 

分 类 号：S643.1[农业科学—蔬菜学]