金鱼草脂氧合酶基因AmLOX1的克隆及表达分析  

Cloning and Expression Analysis of Lipoxygenase Gene AmLOX1 from Snapdragon

作　　者：王少杰[1] 刘晓慧 胡增辉[1] 冷平生[1] Wang Shaojie;Liu Xiaohui;Hu Zenghui;Leng Pingsheng(School of Landscape Architecture, Beijing University of Agricuture, Beijing 102206, China;Beijing Florascape Co. Ltd, Beijing 102206, China)

机构地区：[1]北京农学院园林学院,北京102206 [2]北京市花木有限公司,北京102206

出　　处：《中国细胞生物学学报》2018年第6期905-912,共8页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:31640070);北京市自然科学基金(批准号:8132005);北京市属高等学校创新团队建设与教师职业发展计划项目(批准号:IDHT20150503)资助的课题~~

摘　　要：脂氧合酶(lipoxygenase,LOX)是茉莉酸信号途径的关键酶。该研究克隆了金鱼草的LOX基因,命名为AmLOX1。AmLOX1基因的开放阅读框为2 649 bp,编码883个氨基酸。AmLOX1蛋白质的理论等电点为p H6.06,相对分子质量为100.52 k Da。AmLOX1与其他植物的LOX基因均有较高的同源性。实时荧光定量PCR表达分析发现,AmLOX1在金鱼草花中表达量显著高于根茎叶;在花器官中,下瓣的相对表达量最高;在花不同发育阶段中,衰败期的花AmLOX1的相对表达量最高。Lipoxygenase（LOX） is a key enzyme in jasmonic acid signaling pathway. In this study, the LOX gene of snapdragon was cloned and named as AmLOX1. The open reading frame of the AmLOX is 2 649 bp, which encodes 883 amino acids. The theoretical isoelectric point of AmLOX1 protein is p H6.06, and the relative molecular mass was 100.52 k Da. AmLOX1 shares high homology with the LOX genes of other plants. The Realtime fluorescence quantitative PCR analysis was used to analyze the relative expression of AmLOX1 in snapdragon. The expression of AmLOX1 in snapdragon flower was significant higher than that of root, stem or leaf. While, it expressed highest in lower pedals around the floral organs. And the relative expression level of the decline period was the highest among different developmental stages.

关 键 词：金鱼草 AmLOX1 基因克隆 表达分析 

分 类 号：Q943.2[生物学—植物学]