外碳源投量对亚硝酸型反硝化过程N2O释放影响  

Effect of external carbon source dosage on N_2O emission from denitrification using nitrite as the electron acceptor

作　　者：吕永涛[1] 闫建平 张瑶[1] 刘婷[1] 曾玉莲[1] 王磊[1] LU Yongtao;YAN Jianping;ZHANG Yao;LIU Ting;ZENG Yulian;WANG Lei(School of Environmental and Municipal Engineering,Key Laboratory of Membrane Separation of Shanxi Province, Xi＇ an University of Architecture and Technology,Xi＇ an 710055,China)

机构地区：[1]西安建筑科技大学环境与市政工程学院,陕西省膜分离重点实验室,西安710055

出　　处：《哈尔滨工业大学学报》2018年第8期39-44,共6页Journal of Harbin Institute of Technology

基　　金：国家自然科学基金(51108367); 陕西省建设厅科技发展计划项目(2015-K61)

摘　　要：为实现N_2O的减量化控制或资源化利用,接种普通活性污泥,以乙酸钠和硝酸盐为基质,通过淘洗反硝化聚磷菌,在SBR中以厌氧/缺氧交替运行的方式启动內源反硝化,单周期内约50%的NO-3-N转化为NO-2-N,N_2O释放速率随着亚硝酸的积累逐渐增大,N_2O转化率(释放量占TN去除的比例)为2.04%.在此基础上,分别取缺氧末和厌氧末污泥,对比研究胞内聚合物PHB合成前后,外碳源投加量(碳氮比为0,0.75和2.50)对亚硝酸型反硝化过程N_2O释放特性的影响,结果表明,外碳源存在时,N_2O释放量随总碳源的增加呈略微减少的趋势,转化率在0.24%~1.61%;而当仅利用內源物质进行反硝化时,N_2O的转化率高达15.90%,单位SS最大释放速率达71.29μg/(min·g),释放量是其余条件下的14~26倍.表明单独利用PHB进行亚硝酸型反硝化会大幅增加N_2O的释放.To eliminate N2O emission or utilize it for energy recovery,a laboratory-scale SBR inoculated with common activated sludge with acetate and nitrate additions decoupled,was conducted for endogenous denitrification running with anaerobic/anoxic cycles without DPAOs. During one cycle, approximately 50% of nitrate was converted to nitrite,N_2O emission rate increased with nitrite accumulation,and 2. 04% of removed nitrogen was emitted as nitrous oxide（ N2O）. Batch tests were conducted to compare the emission of N2O that was influenced by external carbon source dosage（ C/N was 0,0. 75 and 2. 50） using nitrite as the electron acceptor before and after poly-β-hydroxybutyrate（ PHB） was synthesized. The results showed that,the N2O emission was slightly decreased with the increase of total carbon source,and the N2O conversion ratio was between 0. 24% and 1. 61%. 15. 90%of N2O conversion ratio was obtained in the absence of external organic carbon,the maximum N2O emission rate was 71. 29 μg/（ min·g）,and the N2O emission was 14-26 times higher than that of the other batch test results,indicating that when the PHB was the sole electron donor,the endogenous denitrification could produce more N2O using nitrite as the electron acceptor.

关 键 词：內源反硝化 碳氮比 N2O PHB 亚硝酸 

分 类 号：X703.1[环境科学与工程—环境工程]