双酚A联合己烯雌酚对未成年雌性SD大鼠的雌激素效应  

Estrogenic effects of bisphenol A combined with diethylstilbestrol in immature female SD rats

作　　者：王娟 刘文龙 赵苒[3] 范春[3] WANG Juan;LIU Wenlong;ZHAO Ran;FAN Chun(Department of Medical Technology,Xiamen Medical College,Xiamen 361023,China;Children Development and Behavior Department,Xiamen Maternity and Child Health Hospital,Xiamen 361003,China;School of Public Health,State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics,Xiamen University,Xiamen 361102,China)

机构地区：[1]厦门医学院医学技术系,福建厦门361023 [2]福建省厦门市妇幼保健院儿童发育行为科,福建厦门361003 [3]厦门大学公共卫生学院分子疫苗学和分子诊断学国家重点实验室,福建厦门361102

出　　处：《吉林大学学报（医学版）》2018年第4期731-735,891,共6页Journal of Jilin University：Medicine Edition

基　　金：福建省教育厅中青年教师教育科研项目资助课题(JAT160582);福建省厦门市科技计划项目资助课题(3502Z20159004);厦门医学院博士基金资助课题(K2016-11)

摘　　要：目的:探讨双酚A(BPA)和己烯雌酚(DES)单独和联合作用于未成年雌性SD大鼠后的雌激素效应,为进一步阐明环境雌激素(EEs)的危害机制提供依据。方法:未成年雌性SD大鼠(21d),分为BPA(200、400和800 mg·kg-1)组,DES(15和150μg·kg-1)组和BPA+DES(100 mg·kg-1 BPA+7.5μg·kg-1 DES,200mg·kg-1 BPA+7.5μg·kg-1 DES,400 mg·kg-1 BPA+7.5μg·kg-1 DES,400mg·kg-1 BPA+75.0μg·kg-1 DES)联合组,同时设对照组;观察各组大鼠子宫湿质量、子宫/体质量比、子宫腔内膜上皮高度、宫腔平滑肌厚度、宫腔腺体数量和成熟卵泡数量。结果:800mg·kg-1 BPA组大鼠子宫湿质量、子宫/体质量比、子宫腔内膜上皮高度、宫腔平滑肌厚度和宫腔腺体数量较对照组明显增加(P<0.05);15和150μg·kg-1 DES组及各剂量联合组大鼠子宫湿质量、子宫/体质量比、子宫腔内膜上皮高度、宫腔平滑肌厚度、宫腔腺体数量和成熟卵泡数量较对照组明显增加(P<0.05),但各剂量联合组大鼠上述指标与各剂量DES组比较差异均无统计学意义(P>0.05)。结论:BPA和DES在单独作用时均具有雌激素活性,BPA联合DES的雌激素效应表现为相加作用。Objective：To investigate the estrogenic effects of BPA and DES co-administered or administered single in the immature female SD rats,and to provide the scientific evidences for further study on the toxicological mechanism of environmental estrogens（EEs）.Methods：The immature female SD rats（21 d）were administered with BPA（200,400,and 800 mg·kg-1）,DES（15 and 150μg·kg-1）and the mixture of BPA and DES（100 mg·kg-1 BPA ＋ 7.5μg·kg-1 DES,200 mg·kg-1 BPA ＋ 7.5μg·kg-1 DES,400 mg·kg-1 BPA＋7.5μg·kg-1 DES,400 mg·kg-1 BPA ＋ 75μg·kg-1 DES）;at the same time,control group was set up.The uterine weights,the ratios of uterine weights to body weights,the heights of luminal epithelium,the thickness of myometrium,the amounts of uterine glands,and the amounts of mature follicle of the rats in various groups were observed.Results：The uterine weight,the ratio of uterine weight to body weight,the height of luminal epithelium,the thickness of myometrium,and the amount of uterine glands of the rats in 800 mg·kg-1 BPA group were increased significantly compared with control group（P〈0.05）.The uterine weights,the ratios of uterine weights to body weights,the heights of luminal epithelium,the thickness of myometrium,the amount of uterine glands and the amounts of mature follicle in 15 and 150μg·kg-1 DES groups were significantly increased compared with control group（P〈0.05）.The uterine weights,the ratios of uterine weights to body weights,the heights of luminal epithelium,the thickness of myometrium,the amounts of uterine glands and the amount of mature follicle in combination groups with different doses were increased significantly（P〈0.05）,but had no changes compared with different doses of DES groups（P〈0.05）.Conclusion：BPA and DES use alone have the estrogenic activities,and the estrogenic effects of co-administered BPA and DES are additive.

关 键 词：双酚A 己烯雌酚 子宫增重实验 雌激素效应 

分 类 号：R994.6[医药卫生—毒理学]