ELK-3蛋白在肝癌组织中的表达及其对肝癌HepG2细胞迁移和侵袭能力的影响  

Expression of ELK-3 protein in hepatocellular carcinoma tissue and its effects on migration and invasion abilities of HepG2 cells

作　　者：李天柱[1] 王加茹 张俊毅[1] 王洪权[1] 史铁伟[1] 周静[1] 白春英[1] 金成浩[2] LI Tianzhu;WANG Jiaru;ZHANG Junyi;WANG Hongquan;SHI Tiewei;ZHOU Jing;BAI Chunying;JIN Chenghao(Molecular Medicine Research Center,College of Medical Sciences,Chifeng University,Chifeng 024000,China;Department of Biochemistry and Molecular Biology,College of Life Science ＆ Technology,Heilongjiang Bayi Agricultural University,Daqing 163319,China)

机构地区：[1]赤峰学院医学院分子医学研究中心,内蒙古赤峰024000 [2]黑龙江八一农垦大学生命科学技术学院生物化学与分子生物学教研室,黑龙江大庆163319

出　　处：《吉林大学学报（医学版）》2018年第4期736-740,892,共6页Journal of Jilin University：Medicine Edition

基　　金：内蒙古自治区科技厅自然科学基金资助课题(2015MS0801)

摘　　要：目的:探讨转录因子ELK-3与肝癌患者临床病理特征的关系及ELK-3对人肝癌HepG2细胞迁移和侵袭能力的影响,并阐明其作用机制。方法:选取80例肝癌患者癌组织及49例癌旁正常组织石蜡标本、18例肝癌患者癌组织及癌旁正常组织新鲜术后标本,采用免疫组织化学法和Western blotting法检测ELK-3蛋白在肝癌及癌旁正常组织中的表达,探讨ELK-3与肝癌患者临床病理特征的关系。选取处于对数生长期的人肝癌HepG2细胞,设立对照组(control-siRNA)和ELK-3干扰组(ELK-3-siRNA),将ELK-3siRNA转染至HepG2细胞,通过细胞划痕实验及体外Transwell小室实验观察肝癌HepG2细胞的迁移和侵袭能力。结果:石蜡组织标本免疫组织化学染色,与癌旁正常组织比较,肝癌组织中ELK-3蛋白阳性表达率明显升高(P<0.05),且ELK-3蛋白阳性表达率与肝癌患者肿瘤数、TNM分期、Edmondson分级和门静脉浸润有关联(P<0.05)。新鲜术后标本Western blotting法检测,与癌旁正常组织比较,18例肝癌患者肝癌组织中ELK-3蛋白表达水平明显升高(P<0.05)。与对照组比较,ELK-3-siRNA组细胞的迁移距离明显变短(P<0.05),侵袭细胞数明显减少(P<0.05)。结论:ELK-3在肝癌组织中的表达水平明显升高。干扰ELK-3会抑制肝癌细胞的迁移和侵袭,表明ELK-3蛋白可以通过抑制肝癌细胞的迁移和侵袭对其产生影响,从而在肝癌发生发展过程中起重要作用。Objective：To explore the relationships between ELK-3 and the clinicopathologic features of the patients with hepatocellular carcinoma（HCC）and the effects of ELK-3 on the migration and invasion abilities of human HCC HepG2 cells,and to clarify their mechanisms.Methods：The paraffin-embedded sections of 80 cases of carcinoma tissue and 49 cases of para-carcinoma tissue,fresh postoperative specimens of 18 cases of carcinoma tissue and para-carcinoma tissue were selected.The expressions of ELK-3 protein in HCC and para-carcinoma normal tissues were detected by immunohistochemistry and Western blotting method.The human HCC HepG2 cells at logarithmic growth phase were divided into control-siRNA and ELK-3-siRNA groups.ELK-3 siRNA was transfected into the HepG2 cells,and the migration and invasion abilities of HepG2 were observed by wound-healing assay and Transwell assay.Results：The immunohistochemistry results showed that compared with the paracarcinoma tissue,the positive expression rate of ELK-3 in the HCC tissue was significantly increased（P〈0.05）,and the positive expression rate of ELK-3 was positively related to the tumor number,TNM stage,Edmondson classification and venous infiltration of the patients（P〈0.05）.The Western blotting results showed that compared with the para-carcinoma tissue,the expression level of ELK-3 protein in the HCC tissue of 18 HCC patients were markedly increased（P〈0.05）.The migration distance and the number of invasion cells in ELK-3-siRNA group were remarkably shorter and less than those in control-siRNA group（P〈0.05）.Conclusion：The expression level of ELK-3 in HCC tissue is significantly increased,and ELK-3 interference could inhibit the migration and invasion of HepG2 cells,which shows that ELK-3 could exert important effects on the occurrence and development of liver cancer by inhibiting the migration and invasion of HCC cells.

关 键 词：转录因子ELK-3 肝肿瘤 HEPG2细胞 细胞迁移 细胞侵袭 

分 类 号：R735.7[医药卫生—肿瘤]