川芎嗪逆转庆大霉素所致豚鼠耳肾氧化应激反应  

Reversal Effect of Tetramethylpyrazine on Gentamicin-induced Oxidative Stress of Ear and Kidney in Guinea Pigs

作　　者：王佳 朱鹏霞 林松柳 药阳洋 倪月秋[1] WANG Jia;ZHU Pengxia;LIN Songliu;YAO Yangyang;NI Yueqiu(Institute of Basic Medicine,Shenyang Medical College,Shenyang 110034,China)

机构地区：[1]沈阳医学院基础医学院,沈阳110034

出　　处：《医药导报》2018年第8期939-942,共4页Herald of Medicine

基　　金：国家自然科学基金资助项目(30672739;81273809);2014年地方高校国家级大学生创新创业训练计划项目(201410164005)

摘　　要：目的研究川芎嗪(TMP)对庆大霉素(GM)耳肾中毒豚鼠听觉脑干诱发电位(ABR)阈值和耳肾组织氧化应激指标的影响。方法选用40只健康白色红目豚鼠,随机分为4组:0.9%氯化钠溶液(NS)组、GM组、TMP组、GM联合TMP混合(GM+TMP)组。4组均连续用药10 d。在给药前和给药10 d后分别检测ABR阈值,停药处死后对4组豚鼠耳蜗、肾组织中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量和谷胱甘肽过氧化物酶(GSH-PX)活性进行检测。结果4组用药前ABR阈值差异无统计学意义。给药10 d后,GM组ABR阈值较给药前发生显著变化(t=2.56,P<0.05),并明显高于对照组(t=3.86,P<0.05);GM+TMP组ABR阈值虽然在停药后也有所增高,但较GM组明显降低(t=2.78,P<0.05)。给药10 d后,豚鼠肾组织中SOD和GSH-PX活性、MDA含量逆转程度与耳蜗比较差异有统计学意义(t=2.45,P<0.05)。结论 TMP通过减少体内活性氧、自由基产生而逆转耳肾氧化应激反应,降低耳肾毒性,并且对肾毒性的逆转作用强于对耳毒性的逆转作用。Objective To study the effects of tetramethylpyrazine（ TMP） on ABR threshold and oxidative stress in gentamicin（ GM）-induced toxicosis of ear and kidney in guinea pig. Methods Forty healthy white guinea pigs were randomly divided into four groups： 0. 9% sodium chloride solution（ NS） group,gentamicin（ GM） group,tetramethylpyrazine（ TMP）group,gentamicin and tetramethylpyrazine（ GM ＋TMP） group. Each group was given TMP for continuous 10 days. The levels of superoxide dismutase（ SOD）,malondialdehyde（ MDA） and glutathione peroxidase（ GSH-PX） were measured in the cochlea and kidney of guinea pigs before and 10 days after dosing. Results There was no significant difference in ABR threshold between each group before administration. After 10 days of administration,the ABR threshold of GM group was significantly changed（ t = 2.56,P〈0.05）. The ABR threshold of GM＋TMP group was significantly lower than that of GM group（ t = 3.86,P〈0.05）. The activity of GSH-PX decreased significantly（ t = 2. 78,P〈0. 05）. The activity of SOD,MDA and GSH-PX in renal tissue of guinea pigs were significantly different from those in cochlea（ t = 2. 45,P〈0. 05）. Conclusion TMP reverses the oxidative stress response by decreasing the production of reactive oxygen species,free radicals and,and reduces the toxicity in the ear and kidney. The reversing effect is stronger on renal toxicity than on ear toxicity.

关 键 词：川芎嗪 庆大霉素 耳毒性 肾毒性 听觉脑干诱发电位 

分 类 号：R285.5[医药卫生—中药学] R978.12[医药卫生—中医学]