微滴技术的数字PCR研究现状及发展趋势  

Research status and trend of digital PCR based on microdroplet technology

作　　者：刘聪 蒋克明[1,2] 周武平 张涛[1,2] 印晨宇 黎海文[1,2] LIU Cong;JIANG Ke-ming(School of Engineering Science,University of Science and Technology of China,Hefei 230022,China;Suzhou Institute of Biomedical Engineering and Technology,Chinese Academy of Sciences,Suzhou 215163,China)

机构地区：[1]中国科学技术大学工程科学学院,安徽合肥230022 [2]中国科学院苏州生物医学工程技术研究所,江苏苏州215163

出　　处：《化学研究与应用》2018年第7期1041-1047,共7页Chemical Research and Application

基　　金：国家重大科学仪器设备开发专项(ZDYZ2013-1)资助; 苏州市科技计划项目(SYG201724)资助; 国家高技术研究发展计划(863计划)项目(2015AA042402)资助

摘　　要：微滴式数字聚合酶链式反应(dd PCR)技术结合了液滴微流控芯片与数字PCR技术,是一种新型的核酸扩增及检测技术。其不仅继承了数字PCR技术高通量、高灵敏度、低消耗等优点,而且解决了传统数字PCR芯片结构复杂、加工困难、成本高等问题,在生命科学领域得到了广泛的应用。本文综述了近年来微滴式数字PCR技术的研究现状,以及目前的发展趋势。Microdroplet digital polymerase chain reaction（ PCR） technology is a new type of nucleic acid amplification and detection technology which combines droplet microfluidic chip and digital PCR technology.It not only inherits the advantages of high throughput,high sensitivity and low consumption of digital PCR technology,but also solves the problems of complex structure,difficult processing and high cost of traditional digital PCR chip.This technology has been widely used in the field of life sciences.This paper reviews the current research situation and development trend of microdroplet digital PCR technology.

关 键 词：数字聚合酶链式反应 液滴 微流控 研究现状 发展趋势 

分 类 号：O657[理学—分析化学]