尿酸在连续碱基DNA-纳米金复合膜修饰电极上的电化学行为及应用研究  

Electrochemical behavior of uric acid at CA-DNA and gold nanoparticles composite modified electrode and its analytical application

作　　者：翟一静[1] 王玮[1] 康维钧[1] 牛凌梅[1] ZHAI Yi-jing;WANG Wei;KANG Wei-jun;NIU Ling-mei(School of public health,Hebei medical university,Shijiazhuang 050017,China)

机构地区：[1]河北医科大学公共卫生学院,河北石家庄050017

出　　处：《化学研究与应用》2018年第7期1075-1080,共6页Chemical Research and Application

基　　金：国家自然科学基金项目(81402720)资助;河北省杰出青年基金项目(H2016206557)资助

摘　　要：构建了连续腺嘌呤碱基的DNA(CA-DNA)与纳米金相结合的尿酸检测传感器。利用扫描电镜对此复合膜进行了表征,并用差分脉冲伏安法研究了尿酸在此修饰电极上的电化学行为。实验发现该修饰电极对尿酸的氧化显示出了明显的电催化性能,浓度在9.00×10^(–8)mol·L^(-1)到9.00×10^(–5)mol·L^(-1)范围内与其峰电流呈良好的线性关系,检出限为3.00×10^(–9)mol·L^(-1)。所述方法方便,低廉,用于人体血清和尿液的测定,获得了较好的结果。A uric acid（ UA） determination biosensor was fabricated using DNA with consecutive adenines（ CA-DNA） and gold nanoparticles（ GNP）. The DNA and GNP composite was characterized through scanning electron microscopy（ SEM）. The electrochemical behavior of UA at this modified electrode was studied by differential pulse voltammetry（ DPV）. It was found that the modified electrode exhibited ulstrasensitive response to UA.The linear relationship between concentration and oxidation peak current was in the range from 9. 00 ×10^-8 mol·L^-1 to 9. 00 ×10^-5 mol·L^-1.The detection limit was 3. 00 ×10^-9 mol·L^-1.The proposed method was low-cost and feasibility and can be applied in the determination of human serum and urine with satisfactory results.

关 键 词：DNA 纳米金 尿酸 金电极 

分 类 号：O657.1[理学—分析化学]