微流控芯片非接触电导法快速测定盐酸多塞平  

Rapid detection of doxepin hydrochloride by microchip capillary electrophoresiswith contactless conductivity detection

作　　者：苏子豪 韦萍 翟海云 徐健君 林敬明[1] SU Zi-hao;WEI Ping;ZHAI Hai-yun;XU Jian-jun;LIN Jing-ming(ZhuJiang Hospital of Southern Medical University,Guangzhou 510280,China;College of Pharmacy,Guangdong Pharmaceutical University,Guangzhou 510006,China;Guangdong Province Zhuhai Municipal Public Security Forensic Science Centre,Zhuhai 519075,China)

机构地区：[1]南方医科大学珠江医院,广东广州510280 [2]广东药科大学药学院,广东广州510006 [3]广东省珠海市公安司法鉴定中心,广东珠海519075

出　　处：《化学研究与应用》2018年第7期1081-1085,共5页Chemical Research and Application

基　　金：南方医科大学科研启动基金项目(PY2017N028)资助

摘　　要：建立了微流控芯片毛细管电泳非接触电导检测法快速测定片剂中盐酸多塞平的方法。探讨了缓冲液类型和浓度、分离电压、进样时间等因素对分离检测的影响。采用4.0 mmol·L^(-1)醋酸-5.0 mmol·L^(-1)醋酸钠(pH=4.78)为缓冲溶液,分离电压2.0 kV,在2.0 min内实现了盐酸多塞平的快速分离测定。在10.0~110.0μg·mL^(-1)范围内,峰面积与浓度呈良好的线性关系,检出限为2.0μg·mL^(-1)(S/N=3),RSD为1.0%,其片剂加标回收率为97.7%~101.6%。该方法快速简便,重现性好,适用于快速测定盐酸多塞平的含量。A method for the rapid determination of doxepin hydrochloride by chip capillary electrophoresis with contactless conductivity detection was built in this article.Effects of the type and concentration of buffer,separation voltage and injection time on the separation and detection were investigated.Doxepin hydrochloride was separated and detected in the buffer solution of 4. 0 mmol·L^-1 HAc-5. 0 mmol·L^-1 Na Ac（ pH = 4. 87） at a separation voltage of 2. 0 kV within 2. 0 mins.The peak area showed a good linear relationship with the concentration of doxepin hydrochloride ranged from 10. 0 μg·mL^-1 to 110. 0 μg·mL^-1,the limit of detection reached 2. 0 μg·mL^-1（ S/N= 3）,the RSD was 1. 0%. The recoveries of tablets were 97. 7% - 101. 6%. The method can be used for the rapid detection of doxepin hydrochloride due to its simplicity,rapidity and reproducibility.

关 键 词：芯片毛细管电泳 非接触电导检测 盐酸多塞平 

分 类 号：O657.1[理学—分析化学]