基于高密度Bin图谱的水稻抽穗期QTL定位  

QTL Mapping for Heading Date in Rice Using High-density Bin Map

作　　者：董骥驰 杨靖[1] 郭涛[1] 陈立凯[1] 陈志强[1] 王慧[1] DONG Ji-Chi;YANG Jing;GUO Tao;CHEN Li-Kai;CHEN Zhi-Qiang;and WANG Hui(National Engineering Research Centre of Plant Space Breeding / South China Agricultural University,Guangzhou 510642,Guangdong,China)

机构地区：[1]华南农业大学/国家植物航天育种工程技术研究中心,广东广州510642

出　　处：《作物学报》2018年第6期938-946,共9页Acta Agronomica Sinica

基　　金：国家现代农业产业技术体系建设专项(CARS-01-12);国家重点研发计划项目(2016YFD0102102);广东省应用型研发项目(2015B020231011)资助~~

摘　　要：以粳稻品种02428和籼稻品种玉针香进行杂交,按单粒传法连续自交10代,得到包含192个株系的重组自交系(RIL)作图群体。通过对两亲本重测序及RIL群体简化基因组测序,构建了包含2711个Bin标记的高密度遗传图谱。该图谱各染色体标记数在162~311个之间,标记间平均物理距离为137.68 kb。将亲本及192个株系分别于4个环境下采用随机区组种植,并记录抽穗期。使用Win QTL Cartographer 2.5软件的CIM分析方法,进行抽穗期相关QTL检测及定位。在4个环境下定位到影响抽穗期的QTL共14个,分布于第1、第2、第3、第7、第8、第9和第10染色体。其中,q HD2.2和q HD10.2能在3个环境中被重复检测到,表型贡献率分别为5.14%~11.15%和5.35%~16.97%,分别能缩短抽穗期1.66 d和1.56 d,具有聚合育种的应用价值。通过物理位置比对,14个QTL中有11个与前人定位在相同或邻近区域,q HD1.1、q HD2.2和q HD9.1尚未见报道。经对q HD2.2详细分析,在其染色体区间内找到3个与抽穗期相关的注释基因LOC_Os02g46450、LOC_Os02g46710和LOC_Os02g46940,其中LOC_Os02g46450已被克隆。测序分析发现,这3个基因在两亲本间都存在差异,可作为候选基因。A recombination inbred lines（RIL） population including 192 lines derived from an inter-subspecific cross between indica rice ‘Yuzhenxiang＇ and japonica rice ‘02428＇ was used in the experiment. The two parent varieties and RIL population were separately sequenced by Whole Genome Sequencing（WGS） and Genotyping-By-Sequencing（GBS） to construct a genetic linkage map with 2711 recombination Bin markers. The number of markers on 12 chromosomes ranged from 162 to 311, and the average physical distance between two markers was 137.68 kb. The Win QTL Cartographer 2.5 was used to analysis QTLs associated with heading date in four different environments. A total of 14 QTLs associated with heading date were detected on chromosomes 1, 2, 3, 7, 8, 9, and 10. Among them, q HD2.2 and q HD10.2, which explained 5.14%-11.15% and 5.35%-16.97% of the total phenotypic variation for heading date separately, could be detected in three environments and shorting heading date about 1.66 days and 1.56 days on average. The two QTLs could inherit stablely, having a good potential to be applied in QTL pyramiding. After comparing the physical positions of these QTLs with those previously reported, we found 11 QTLs were located in the same or near position, among them q HD1.1, q HD2.2, and q HD9.1 were newly reported. Furthermore, we found one cloned gene LOC_Os02 g46450 and two annotated genes LOC_Os02 g46710 and LOC_Os02 g46940 in the genomic region of q HD2.2, might be related to heading date. DNA sequence comparison between YZX and 02428 revealed that all the three genes could be candidate genes.

关 键 词：水稻 抽穗期 Bin图谱 QTL定位 

分 类 号：S511[农业科学—作物学]