载气吹扫尼古丁长期吸入对大鼠心血管功能、氧化应激和炎症水平影响  

Effects of long-term inhalation of nicotine by gas-purge on cardiovascular function,oxidative stress and inflammation in rats

作　　者：程攀[1] 何庆[2,3] 张晓宇[2,3] 张永学 张文欣 汪志伟 赵庆中 高杉[1] Cheng Pan;He Qing;Zhang Xiaoyu(Dept of Pharmacology,Anhui Medical University,Hefei 230032;Combustion ＆ Pyrolysis Studyof Key Laboratory of China Tobacco,China Tobacco Anhui Industrial Co.,Ltd.,Hefei 230088;Anhui Key Laboratory of Tobacco Chemistry,China Tobacco Anhui Industrial Co.,Ltd.,Hefei 230088)

机构地区：[1]安徽医科大学药理学教研室,合肥230032 [2]烟草行业燃烧热解研究重点实验室(安徽中烟工业有限责任公司),合肥230088 [3]烟草化学安徽省重点实验室(安徽中烟工业有限责任公司),合肥230088

出　　处：《安徽医科大学学报》2018年第6期834-840,共7页Acta Universitatis Medicinalis Anhui

基　　金：烟草化学安徽省重点实验室开放课题(编号:2015034);科技部重大新药创制资助课题(编号:2017ZX09301012)

摘　　要：目的研究载气吹扫尼古丁吸入对大鼠心血管功能、氧化应激和炎症水平的影响并初步探讨其机制。方法将40只雄性健康SD大鼠随机均分为4组。实验组进行载气吹扫尼古丁法致大鼠吸入,每日分别定时暴露1、2、4 h,确定为低、中、高剂量组。对照组在标准条件下饲养。90 d后,测定大鼠心功能,心、肺、血清中内皮型一氧化氮合酶(eNOS)、白介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)、白介素10(IL-10)含量,血清中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果与对照组比较,暴露处理组血压明显升高,血清SOD活性降低,谷胱甘肽过氧化物酶(GSH-Px)含量升高,而MDA含量明显降低。心、肺血清IL-1β、TNF-α、IL-10含量均有不同程度的升高。结论载气吹扫尼古丁吸入的大鼠血压升高,心功能存在障碍,机体氧化-抗氧化失衡并且心肺发生炎症反应,其机制可能与内皮细胞功能紊乱和交感、副交感系统的同时激活有关。Objective To investigate the effects of inhalation of nicotine on cardiovascular function,oxidative stress and inflammation in rats and its mechanism. Methods Forty healthy male SD rats were randomly divided into four groups. The experimental group was inhaled nicotine by air-purging. The rats were exposed for 1 h,2 h and 4 h daily,respectively,and were determined as low,medium and high dose groups. The control group was housed under standard conditions. After 90 days,aortic reactivity and blood pressure were measured. The levels of endothelial NO synthase（ eNOS）,interleukin-1β（ IL-1β）,tumor necrosis factor-α（ TNF-α） and interleukin-10（ IL-10）in heart,lung tissue and serum of rats were measured. Serum nitric oxide（ NO） content,malondialdehyde（ MDA） content,superoxide dismutase（ SOD） activity and glutathione peroxidase（ GSH-Px） activity were measured respectively. Results Compared with the control group,the blood pressure of the experimental group was significantly increased. NO,eNOS and MDA content were significantly decreased. GSH-Px activity was significantly increased. The levels of IL-1β,TNF-α,IL-10 were significantly increased in heart,lung and serum respectively.Conclusion Long-term inhaled nicotine by air-purged impaired cardiac function and caused high blood pressure,induced oxidative stress and cardiopulmonary inflammatory reaction. The mechanism may be related to endothelial dysfunction and simultaneous activation of sympathetic and parasympathetic systems.

关 键 词：尼古丁 心血管功能 氧化应激 炎症因子 大鼠 

分 类 号：R965[医药卫生—药理学]