芹菜素改善肥胖大鼠肾脏氧化应激损伤  

Apigenin improves oxidative stress damage in obese rats

作　　者：黄斌 陶小根 陈剑 陈超 叶山东 Huang Bin;Tao Xiaogen;Chen Jian(Dept of Endocrinology;Dept of Critical Care Medicine,The Affiliated Provincial Hospital of Anhui Medical University,Hefei 230001)

机构地区：[1]安徽医科大学附属省立医院内分泌科,合肥230001 [2]安徽医科大学附属省立医院重症医学科,合肥230001

出　　处：《安徽医科大学学报》2018年第6期856-860,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金面上项目(编号:11040606M159)

摘　　要：目的研究芹菜素对肥胖大鼠肾脏代谢状态的影响以及可能机制。方法 4周龄雄性SD大鼠按随机数字表法分为对照组和高脂组。高脂组予连续高脂喂养12周后,再随机分为肥胖组、干预组。对照组和肥胖组予0.9%生理盐水灌胃,干预组予芹菜素40 mg/(kg·d)灌胃处理,4周后通过Western blot、DHE探针染色和免疫组化检测肾脏组织中氧化应激、炎症和自噬相关指标。结果 (1)芹菜素干预4周后,与肥胖组相比,芹菜素组体重有一定的下降趋势,但是差异无统计学意义(t=1.80,P=0.096)。(2)DHE探针染色检测肥胖大鼠肾脏示O_2-较对照组明显增多(t=-3.58,P=0.023),经过4周菜素治疗后明显好转(t=2.79,P=0.049)。(3)肥胖大鼠肾脏p-P67、p-P65等表达明显增加,芹菜素干预后,其蛋白水平较肥胖组(均P<0.05)出现显著下降。(4)经芹菜素治疗后肾脏自噬相关蛋白LC3BII/LC3BI、Beclin1水平明显上升,与肥胖组相比差异有统计学意义(均P<0.05)。结论芹菜素可明显改善肥胖大鼠肾脏内氧化应激、炎症的代谢紊乱状态,可能与其激活自噬通路有关。Objective To investigate the effects of apigenin on renal metabolic status and its possible mechanism in obese rats. Methods All 4-week-oldmale SD rats were randomly divided into Con group and HFD group. The HFD group was fed with high-fat diet for 12 weeks,then divided into Apigenin group and Obesity group randomly.The Apigenin group wastreated with apigenin 40 mg/（ kg·d） for another 4 weeks. The Con and Obesity groups of rats were received 0. 9% normal saline in the meantime. Western blot and immunohistochemical were used for protein expression assays. Results（1） After 4 weeks apigenin treatment,the rat weights were decreased compared with Obesity group（ t = 1. 80,P = 0. 096）,but the difference was not statistically significant.（2） DHE staining was used to evaluate the O2-formation in the kidney. In apigenin group,the content of O2-was decreased distinctly compared with Obesity（ t = 2. 79,P = 0. 049）.（3） The protein expression of p-P67 and p-p65 were decreased compared with Obesity（ P 〈 0. 05） after apigenin treatment.（4） LC3 BII/LC3 BI and Beclin1 protein levels in kidney were increased significantly compared with the other two groups after apigenin treatment（ P 〈 0. 05）. Conclusion Apigenin alleviates the obese rats renal oxidative stress and inflammation state,which may be related to the activation of autophagy pathway.

关 键 词：芹菜素 肥胖 自噬 氧化应激 炎症 

分 类 号：R589.2[医药卫生—内分泌]