普伐他汀钠对LPS诱导的中性粒细胞NE释放及活性的影响  

Pravastatin sodium inhibits the release and activity of human peripheral blood neutrophil elastase induced by lipopolysaccharide

作　　者：陈雨虹[1] 戴敏 梅晓冬[1] Chen Yuhong;Dai Min;Mei Xiaodong(Dept of Respiratory Medicine,The Affiliated Provincial Hospital of Anhui Medical University,Hefei 230001)

机构地区：[1]安徽医科大学附属安徽省立医院呼吸内科,合肥230001

出　　处：《安徽医科大学学报》2018年第6期880-884,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:1308085MH115)

摘　　要：目的研究普伐他汀钠对细菌脂多糖(LPS)诱导的人外周血中性粒细胞弹性蛋白酶(NE)释放及活性的影响。方法采用Ficoll密度梯度离心法分离培养人外周血中性粒细胞,瑞氏染色法鉴定中性粒细胞及胞内嗜天青颗粒,使用LPS刺激中性粒细胞脱颗粒,提取细胞上清液,应用比色法检测上清液中过氧化物酶(MPO)活性以确定合适的刺激时间和浓度后,给予中性粒细胞LPS刺激同时予以普伐他汀钠处理,使用ELISA法检测细胞培养上清液NE含量,比色法检测NE活性。结果 LPS刺激后中性粒细胞培养上清液中NE的含量和活性均明显增高。普伐他汀钠处理后的细胞培养上清液中NE明显降低(P<0.05)。结论普伐他汀钠能降低LPS刺激导致的中性粒细胞脱颗粒,降低NE释放量及其活性。Objective To study the effects of pravastatin sodium on the release and activity of neutrophil elastase（ NE） in human neutrophile granulocyte induced by lipopolysaccharide（ LPS）. Methods Human neutrophile granulocyte were isolated by Ficoll density gradient centrifugation,neutrophils and intracellular azurophilic granules were identified by myeloperoxidase（ MPO） staining,LPS stimulated degranulation of neutrophils. The MPO activity in the supernatant was measured by colorimetric assay to determine the appropriate stimulus time and concentration,and neutrophil was treated with pravastatin sodium after LPS stimulation. ELISA mothed was used to detect the cell culture supernatant of NE content and NE activity was detected by colorimetric method. Results The content and activity of NE in the supernatant were significantly increased after LPS stimulation. The NE in the supernatant of the cells treated with pravastatin was significantly lower（ P 〈 0. 05）. Conclusion Pravastatin sodium can reduce neutrophil degranulation induced by LPS stimulation and decrease the release and activity of NE.

关 键 词：普伐他汀钠 中性粒细胞弹性蛋白酶 脂多糖 过氧化物酶 

分 类 号：R452[医药卫生—治疗学]