降脂通络软胶囊人血浆药物浓度的测定  

Determination of Drug Concentration in Human Plasma of Jiangzhi Tongluo Soft Capsule

作　　者：戴博 张华峰[1] 戴婷[2] 王健康[1] 曹菊林 Dai Bo;Zhang Huafeng;Dai Ting;Wang Jiankang;Cao Julin(General Hospital of the Air Foree,Beijing 100142,China;Sino Japan Friendship Hospital of Jilin University,Chuangehun 130033,China;ShenWei Pharmaceutical Group limited company,Shijiazhuan 051430,China)

机构地区：[1]空军总医院,北京100142 [2]吉林大学中日联谊医院,吉林长春130033 [3]神威药业集团有限公司,河北石家庄051430

出　　处：《亚太传统医药》2018年第7期63-66,共4页Asia-Pacific Traditional Medicine

摘　　要：目的:建立高效液相色谱-串联质谱法对降脂通络软胶囊人血浆药物浓度进行测定。方法:采用C_(18)色谱柱(3.5μm,50mm×2mm),以乙腈0.1%甲酸进行梯度洗脱,流速:1.0mL·min^(-1)。结果:人血浆内源性杂质对样品测定无干扰,姜黄素、去甲氧基姜黄素、双去甲氧基姜黄素在100～10 000ng·mL^(-1)范围内线性关系良好,最低检测限和最低定量限分别为10ng·mL^(-1)和30ng·mL^(-1),低中高3种浓度的平均回收率均大于80%,日内精密度RSD均小于3%,日间精密度RSD均小于15%。结论:该方法快速、灵敏、准确,可用于降脂通络软胶囊人血浆药物浓度的测定。Objective ： To establish a high performance liquid chromatography-tandem mass spectrometry method for the determination of human plasma drug concentration in Jiangzhi Tongluo Soft Capsule. Methods ： C18 column was eluted with acetonitrile ： 0.1 formic acid at a flow rate of 1.0 mL · min^-1. Results： Human plasma endogenous impurities on the sample without interference, eurcumin, demethoxy curcumin, didekylated curcumin at 100-10000ng·mL^-1 in a linear relationship . The minimum detection limit and the minimum limit of quantification are 10ng·mL^-1 and 30ng·mL^-1, the average recoveries of the three medium concentrations were higher than 80%, the intra day precision RSD was less than 3%, and the intra day precision RSD was less than 15%. Conclusion：This method is rapid, sensitive and accurate, can be used for lipid lowering Tongluo soft capsule plasma plasma con centration determination.

关 键 词：降脂通络软胶囊 高效液相色谱-串联质谱法 血浆药物浓度 姜黄素 去甲氧基姜黄素 双去甲氧基姜黄素 

分 类 号：R284.2[医药卫生—中药学]