紫丁香与羽叶丁香叶绿体DNA提取方法研究  

Extraction Method of Chloroplast DNA of Syringa oblata and Syringa pinnatifolia

作　　者：张靖雯 姜在民[2] 蔡靖 ZHANG Jing-wen;JIANG Zai-min;CAI Jing(College of Forestry,Northwest A ＆ F University,Yangling,Shaanxi 712100,China;College of Life Sciences,Northwest A ＆ F University,Yangling,Shaanxi 712100,China)

机构地区：[1]西北农林科技大学林学院,陕西杨陵712100 [2]西北农林科技大学生命科学学院,陕西杨陵712100

出　　处：《西北林学院学报》2018年第4期95-99,共5页Journal of Northwest Forestry University

基　　金：国家林业局林业公益性行业科研专项项目(201204308)

摘　　要：为了探究适合丁香属植物的叶绿体DNA(cpDNA)的提取方法,采取高盐-低pH法和改良高盐-低pH法分别分离提取了紫丁香与羽叶丁香的叶绿体及cpDNA。结果表明,采取高盐-低pH法提取出的cpDNA,其OD260/OD280值均<1.7,且琼脂糖凝胶电泳检测无条带,表明cpDNA质量低,不能满足后续叶绿体基因组测序要求,改良高盐-低pH法提取出的cpDNA,其OD260/OD280值在1.8~1.9,琼脂糖凝胶电泳检测显示条带清晰,无降解现象,表明cpDNA质量高,能够满足后续叶绿体基因组测序要求。研究表明,改良高盐-低pH法可简便快速的提取紫丁香与羽叶丁香的cpDNA,为进一步研究丁香属植物的叶绿体基因组奠定了基础。To explore the suitable extraction method of chloroplast DNA（cpDNA）of SyringaL.,the chloroplast and cpDNA of Syringa oblata and S.pinnatifolia were isolated and extracted by high salt-low pH method and modified high salt-low pH method,respectively.The results showed that the value of OD260/OD280 of cpDNA extracted by the high salt-low pH method was less than 1.7,and agarose gel electrophoresis showed no cpDNA stripes,which indicated that the cpDNA was of low quality and could not meet the requirements of subsequent chloroplast genome sequencing.The value of OD260/OD280 of cpDNA extracted by the modified high salt-low pH method was between 1.8 and 1.9,and agarose gel electrophoresis showed that the cpDNA stripes were bright and no degradation,which indicated that the cpDNA was of high quality and could meet the requirements of subsequent chloroplast genome sequencing.This research showed that the cpDNA of S.oblataand S.pinnatifoliacould be simply and quickly obtained by the modified high salt-low pH method,it laid the foundation for the further study of chloroplast genome of Syringa.

关 键 词：紫丁香 羽叶丁香 叶绿体分离 cpDNA提取 改良高盐-低pH法 

分 类 号：S685.26[农业科学—观赏园艺]