炎性因子对人口腔黏膜上皮细胞β-防御素表达的影响  

Effect of inflammatory cytokines on the expression of beta defensins in human oral mucosa epithelial cells

作　　者：陈怡 刘贞敏[1,3] 雍翔智 蒋兰岚[1,2,3] 吴恬恬 陶人川 CHEN Yi;LIU Zhen-min;YONG Xiang-zhi;JIANG Lan-lan;WU Tian-tian;TAO Ren-chuan(Department of Periodontics and Oral Medicine,Stomatological Hospital,Guangxi Medical University,Nanning 530021,China;Guangxi Key Laboratory of AIDS Prevention and Treatment,Nanning 530021,China;Guangxi Key Laboratory of Oral and Maxillofacial Repair and Reconstruction Research,Guangxi Key Laboratory of Diagnosis and Treatment of Maxillofacial Surgery Diseases,Nanning 530021,China)

机构地区：[1]广西医科大学附属口腔医院牙周黏膜科,南宁市530021 [2]广西艾滋病防治研究中心重点实验室,南宁市530021 [3]广西口腔颌面修复与重建研究重点实验室广西颌面外科疾病诊治研究重点实验室,南宁市530021

出　　处：《广西医学》2018年第10期1182-1185,共4页Guangxi Medical Journal

基　　金：国家自然科学基金(81260167);广西自然科学基金重点项目(2015GXNSFDA139018)

摘　　要：目的探讨炎性因子对人口腔黏膜上皮细胞(OMEC)人β防御素(hBD)-2和hBD-3表达的影响。方法收集人健康口腔黏膜组织,进行消化培养后选用第2代人OMEC。培养48 h后将OMEC分为空白对照组、肿瘤坏死因子-α(TNF-α)组、干扰素-γ(IFN-γ)组、白细胞介素-17(IL-17)组、TNF-α+IFN-γ组、TNF-α+IL-17组和IFN-γ+IL-17组,除空白对照组外加入相应的细胞因子。干预24 h后采用实时荧光定量PCR检测OMEC的hBD-2、hBD-3 mRNA相对表达量,酶联免疫吸附试法检测细胞上清hBD-2和hBD-3的蛋白水平。结果 TNF-α组hBD-2 mRNA的相对表达量、IL-17组的hBD-2及hBD-3 mRNA的相对表达量均高于空白对照组(均P<0.05),TNF-α+IFN-γ组、TNF-α+IL-17组、IFN-γ+IL-17组hBD-2、hBD-3 mRNA的相对表达量及hBD-2蛋白水平均高于相应细胞因子单独干预组(均P<0.05)。结论炎性因子能诱导人口腔黏膜上皮细胞表达hBD-2和hBD-3。Objective To investigate the effect of inflammatory cytokines on the expressions of human beta-defensin（ hBD）-2 and hBD-3 in human oral mucosal epithelial cell（ OMEC）. Methods Oral mucosal tissues of healthy subjects were collected. The second-generation human OMECs were selected after digestion and culture. After 48 hours of culture,the OMECs were divided into blank control group,tumor necrosis factors-α（ TNF-α） group,interferon-γ（ IFN-γ） group,interleukin-17（ IL-17） group,TNF-α ＋ IFN-γ group,TNF-α ＋ IL-17 group and IFN-γ ＋ IL-17 group. Then the corresponding cytokines were added to all groups except the blank control group. After 24 hours of intervention,the relative expression levels of hBD-2 and hBD-3 mRNAs in OMECs were detected by real-time quantitative PCR,and enzyme-linked immunosorbent assay was used to detect the protein levels of hBD-2 and hBD-3 in cell supernatant. Results Compared to the blank control group,the relative expression level of hBD-2 mRNA in the TNF-α group and the relative expression levels of hBD-2 and hBD-3 mRNAs in the IL-17 group were higher（ all P〈0. 05）. The relative expression levels of hBD-2 and hBD-3 mRNAs and the hBD-2 protein levels in the TNF-α ＋ IFN-γ group,TNF-α ＋ IL-17 group and IFN-γ ＋ IL-17 group were higher than those in the group with corresponding single intervention（ all P〈0. 05）. Conclusion Inflammatory cytokines can induce the expressions of hBD-2 and hBD-3 in human OMECs.

关 键 词：炎性因子 Β防御素-2 β防御素-3 人口腔黏膜上皮细胞 

分 类 号：R781.5[医药卫生—口腔医学]