GEB-800尿干化学分析仪法与UF-1000i尿有形成分分析仪法联合检测尿液标本复检规则的探讨  

Discussion on reexamination rules for the combined detection of GEB-800 urine dry chemical analyzer and UF-1000i urine tangible component analyzer for urine specimen

作　　者：王宇[1] WANG Yu(Department of Clinical Laboratory,the Hospital of Civil Aviation Flight University of China,Sichuan Province,Guanghan 618307,China)

机构地区：[1]中国民航飞行学院医院检验科,四川广汉618307

出　　处：《中国当代医药》2018年第15期130-132,139,共4页China Modern Medicine

摘　　要：目的建立和评价GEB-800尿干化学分析仪法与UF-1000i尿有形成分分析仪法联合检测尿液标本的复检规则。方法收集2018年3月中国民航飞行学院医院的1000例尿液分析标本,采用GEB-800尿干化学分析仪法和UF-1000i尿有形成分分析仪法进行尿液干化学和尿液有形成分联合检测。采用双盲法进行离心显微镜复检,以显微镜检查结果均值作为判断标准。设计三种方案用于复检规则优化。方案1:尿干化学检测白细胞酯酶(LEU)、隐血(BLD)、亚硝酸盐(NIT)、蛋白(Pro)任意一项指标阳性。方案2:尿有形成分分析白细胞(WBC)、红细胞(RBC)、管型(Cast)任意一项指标超出参考区间上限。方案3:尿干化学检测LEU、BLD、NIT、Pro结果分别与尿有形成分分析WBC、RBC、CAST结果不符。结果以人工显微镜结果为标准,1000例用于建立复检规则的尿液样本中,总阳性率为39.5%(395/1000),RBC、WBC、Pro、Cast阳性率分别为18.2%(182/1000)、10.6%(106/1000)、9.2%(92/1000)、1.5%(15/1000)。其中,复检方案3的真阳性率为95.5%、假阴性率为4.5%、真阴性率为74.2%、假阳性率25.8%、复检率为22.4%,符合率为82.8%。结论 GEB-800尿干化学分析仪法与UF-1000i尿有形成分分析仪法联合检测尿液标本复检规则最优方案为尿干化学检测LEU、BLD、NIT、Pro的结果分别与尿有形成分分析WBC、RBC、Cast的结果不符。Objective To establish and evaluate the reexamination rules for the combined detection of GEB-800 urine dry chemical analyzer and UF-1000 i urine tangible component analyzer for urine specimen.Methods A total of 1000 urine samples from the hospital of Civil Aviation Flight University of China in March 2018 were collected for study.GEB-800 urine dry chemistry analyzer and UF-1000 i urine tangible component analyzer were used to detect the dry chemistry markers and urine visible components,respectively.The doubly blind method was used for the reexamination of centrifuge microscope,and the mean values of the microscopic examination results were used as the criterion.Three schemes were designed for optimization of reexamination rules.Scheme 1：any positive result of leukocyte esterase（LEU）,occult blood（BLD）,nitrite（NIT）,protein（Pro） by urine dry chemical analyzer.Scheme 2：the urine tangible component analysis any index of leucocyte（WBC）,red blood cell（RBC）,tube type（Cast） exceeded the upper limit of the reference interval.Scheme 3：urine dry chemical detection results of LEU,BLD,NIT and Pro were not consistent with the results of urinary sediment analysis of WBC,RBC,and Cast.Results Using the microscope results as the standard,in the 1000 cases of urine samples for establishment of reexamination rules,the total positive rate accounted for 39.5%（395/1000）,and that of RBC,WBC,Pro and Cast was 18.2%（182/1000）,10.6%（106/1000）,9.2%（92/1000）,1.5%（15/1 000）,respectively.Among them,the true positive rate of the reexamination scheme 3 was 95.5%,the false negative rate was 4.5%,the true negative rate was 74.2%,the false positive rate was 25.8%,the reexamination rate is 22.4%,and the coincidence rate is82.8%.Conclusion The optimal reexamination rule of combined detection by GEB-800 urine dry chemistry analyzer and UF-1000 i urine tangible component analyzer is that urine dry chemical detection results of LEU,BLD,NIT and Pro are not consistent with the results of urinary sedime

关 键 词：尿液 GEB-800尿液干化学分析仪 UF-1000i尿液有形成分分析仪 复检规则 

分 类 号：R446.12[医药卫生—诊断学]