山银花黄酮粗提物对急性心肌缺血氧化损伤大鼠的保护作用  

Effects of flavonoids crude extract from Lonicerae Flos on acute myocardial ischemia in rats

作　　者：李冲[1] 文翔昊[1] 郭露[1] 孙林军 田勇 郭玉[2] LI Chong;WEN Xianghao;GUO Lu;SUN Linjun;TIAN Yong;GUO Yu(Nanhua Affiliated Hospital of University of South China,Hengyang,Hunan 421002;1nstitute of Pharmacy and Phar-macology,University of South China,Hengyang,Hunan 421001,China)

机构地区：[1]南华大学附属南华医院,湖南省衡阳市421002 [2]南华大学药物药理研究所,湖南省衡阳市421001

出　　处：《中国动脉硬化杂志》2018年第8期779-783,835,共6页Chinese Journal of Arteriosclerosis

基　　金：湖南省大学生研究性学习和创新性实验计划项目(2015226)

摘　　要：目的研究山银花黄酮粗提物对急性心肌缺血氧化损伤大鼠的保护作用。方法盐酸异丙肾上腺素皮下注射制备大鼠急性心肌缺血氧化损伤模型。连续灌胃不同浓度山银花黄酮粗提物7天,动态心电记录大鼠左心室压、心电图变化,硝基四氮唑蓝法观测心肌缺血面积百分率,酶联免疫吸附法检测血清中蛋白酶的活性或产物的量。结果以山银花黄酮粗提物喂养的急性心肌缺血大鼠,心电图因异丙肾上腺素引起的ST段上抬、T波升高有明显的缓解,QT间期时程缩短,心肌缺血面积百分率降低,心率、舒张压、左心室收缩压和平均压均有回升;且其血清丙二醛含量减少,肌酸激酶和乳酸脱氢酶活性降低,超氧化物歧化酶活性回升。结论山银花黄酮粗提物具有改善异丙肾上腺素所致大鼠急性心肌缺血氧化损伤的作用。Aim To investigate the protective effects of flavonoid crude extracts fromLonicerae Flos on acute myocardial ischemia in rats. Methods The rat model of acute myocardial ischemia（ AMI） in oxidative damage was prepared by subcutaneous injection of isoproterenol hydrochloride. After intragastric administration for 7 days,the changes of rat left ventricular pressure and electrocardiogram were detected by dynamic electrocardiogram,the percentage of myocardial ischemia area was observed by nitroblue tetrazol（ NBT） staining and the activity of protease or the amount of product in serum were detected by ELISA. Results The acute myocardial ischemia rats fed with honeysuckle flavonoids extract,the ST-segment and T wave in their electrocardiogram elevation by isoproterenol-induced were significantly alleviated,and the time of QT interval was shortened. The percentage of myocardial ischemia area was decreased.The heart rate,the left ventricular systolic pressure,diastolic and mean pressure were rebounded significantly. The serum malondialdehyde（ MDA） content was reduced,the activity of protease was back to normal trend with reduction of creatine kinase（ CK） and lacate dehydrogenase（ LDH） and enhancement of superoxide dismutase（ SOD）. Conclusion This study shows that improvement effects of flavonoids crude extract fromLonicerae Flos were confirmed on acute myocardial ischemia caused by oxidative damage in rats.

关 键 词：山银花黄酮粗提物 急性心肌缺血 氧化损伤 

分 类 号：R96[医药卫生—药理学]