5个产地菘蓝种子萌发及幼苗生长对盐胁迫的响应  

Seed germination and seedling growth responses of Isatis indigotica in five populations from saline environments

作　　者：王雨[1] 周睿颖 马立敏[1] 白钰[1] 关佳莉 唐晓清[1] WANG Yu;ZHOU Rui -ying;MA Li -min;BAI Yu;GUAN Jia -li;TANG Xiao -qing(Institute of Chinese Medicinal ,Naterials, Nanjing Agricultural University, Nanjing 210095, China)

机构地区：[1]南京农业大学,中药研究所,江苏南京210095

出　　处：《草业学报》2018年第7期145-154,共10页Acta Prataculturae Sinica

基　　金：国家自然科学基金项目(31171486)资助

摘　　要：以来自河北保定(HB)、内蒙古包头(NB)、辽宁铁岭(LT)、安徽亳州(AH)、山东滨州(SB)5个产地的菘蓝种子为材料,探讨不同浓度0,50,100,150mmol·L-1 NaCl胁迫下各产地菘蓝种子萌发及幼苗生长的变化规律,并综合分析其耐盐性。结果表明,不同产地菘蓝种子的耐盐性存在显著差异。随盐胁迫浓度的升高,5个产地菘蓝种子的萌发率、发芽势、活力指数均整体逐渐降低,50mmol·L-1 NaCl促进了HB、SB幼苗鲜重、下胚轴及胚根长度的增加。100mmol·L-1 NaCl胁迫下,5个产地菘蓝的种子苗鲜重、下胚轴及胚根长度均呈不同程度的降低,丙二醛(MDA)含量显著增加,盐胁迫抑制作用明显。随着盐浓度的升高,LT和AH菘蓝幼苗叶片和根的鲜干重均整体呈下降趋势,5个产地菘蓝幼苗的超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、脯氨酸含量均呈先上升后下降趋势,并在100mmol·L-1 NaCl处理下达到最大值。应用隶属函数法对5个产地菘蓝种子萌发期的耐盐性进行综合评价,其耐盐性由强到弱的排列顺序为:HB>SB>LT>NB>AH。Seedlings of Isatis indigotica from five localities[Baoding,Hebei（HB）;Baotou,Inner Mongolia（NB）;Tie ling,Liaoning（LT）;Bozhou,Anhui（AH）;Binzhou,Shandong（SB）]were used as research materials,to investigate the seed germination and seedling growth of I.indigotica subjected to pure water（0 mmol·L-1 NaCl）or three concentrations of NaCl solution（0,50,100,150 mmol NaCl·L-1）.Salt tolerance among the five populations differed significantly.In general,the germination rate,germination potential,germination index and vitality index decreased with increase in NaCl concentration.NaCl at 50 mmol·L-1 promoted the fresh weight,hypocotyl length and radicle length of I.indigoticaseedlings from the HB and SB localities.The same three traits were significantly decreased in seedlings from all five localities under 100 mmol·L-1 NaCl stress,and malondialdehyde（MDA）concentration increased greatly.The fresh and dry weights of leaf laminae and roots of I.indigotica seedlings from LT and AH localities area were decreased under salt stress at 150 mmol·L-1 NaCl.With increasing NaCl concentration,the activities of superoxide dismutase（SOD）and peroxidase（POD）and proline contents of I.indigoticaseedlings from the five localities increased to a peak at 100 mmol·L-1 NaCl,with values decreasing somewhat at the 150 mmol·L-1 NaCl concentration.From a comprehensive appraisal of membership function method,the salt resistance of I.indigoticaseeds from the five different localities was ranked：HBSBLTNBAH.

关 键 词：菘蓝 盐胁迫 种子萌发 生理特性 隶属函数 

分 类 号：S567.239[农业科学—中草药栽培]