四川不同产地厚朴HPLC指纹图谱及含量测定研究  

Determination and Fingerprint Analysis of Magnolia officinalis Rehd.et Wils from Different Regions in Sichuan Province

作　　者：任波[1] 董佳悦 安太勇 刘美琳[1] 张梅[1] REN Bo;DONG Jia-yue;AN Tai-yong;LIU Mei-lin;ZHANG Mei(State Key Laboratory Breeding Base of Systematic Research,Development and Utilization of Chinese MedicineResources,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,Sichuan,China)

机构地区：[1]成都中医药大学中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137

出　　处：《食品研究与开发》2018年第15期128-134,共7页Food Research and Development

基　　金：四川省科技计划项目"中药药效物质基础研究与创新中药发现四川省青年科技创新研究团队"(2016TD0006)

摘　　要：建立四川不同产地厚朴多波长切换高效液相色谱法(high efficiency liquid chromatography,HPLC)指纹图谱,对厚朴中紫丁香苷、木兰花碱、厚朴酚及和厚朴酚等化学成分进行指认,同时测定厚朴酚、和厚朴酚的含量。采用HPLC-二极管阵列检测器(diode array detector,DAD)方法,Inert Sustain C18(4.6 mm×250 mm,5 m)色谱柱,流动相为乙腈-0.1%磷酸水,梯度洗脱;检测波长为0~31 min:265 nm,31 min^60 min:294 nm;流速0.8 m L/min,柱温35℃。进样量20μL。采用多波长切换技术建立四川厚朴HPLC指纹图谱共有模式,标定了9个共有峰,其中在265 nm下标定2个共有峰为紫丁香苷、木兰花碱;在294 nm下标定2个共有峰为厚朴酚、和厚朴酚;42批四川不同产地厚朴样品相似度为0.868~0.990,说明多批次样品具有一致性;厚朴酚、和厚朴酚总含量范围分别为14.36 mg/g^65.58 mg/g,其中除峨眉山市丰收村、茶地村、乐山市刘沟村、麻柳乡及阿坝州的厚朴中厚朴酚、和厚朴酚总含量较低外,其他产地中厚朴酚、和厚朴酚总含量均大于2.0%,符合药典要求。该方法快捷、有效和可靠,能全面体现特征吸收波长相差较大的多个成分的指纹信息,为有效提高厚朴药材质量控制提供参考。Establish the multi -wavelength chromatographic fingerprints of Magnolia officinalis produced insichuan province,identify the peaks of syringoside,magnoflorine, magnolol and honokiol and determine thecontents of magnolol and honokiol.HPLC -diode array detector was used to establish fingerprints withInertSustain C18 （4.6 mm×250 mm, 5m）column and mobile phase of acetonitrile-0.1 % phosphoric acidsolution mixture,flow rate 0.8 mL/min; wavelength： 0-31 min：265 nm;31 min -60 min：294 nm. Columntemperature：35 ℃; sample size：20μL. After the mutual mode of HPLC fingerprint of the Mangnolia officinalisfrom Sichuan province were set up,9 peaks were calibrated,two common peaks （syringoside andmagnoflorine）were pointed out at 265 nm,two common peaks （magnolol and honokiol） . The 42 batches ofsamples similarity was 0.868-0.990, which Indicated that multiple batches of samples were consistent. In the 42batch of samples,The total content range of magnolol and honokiol were 14.36 mg/g-65.58 mg/g.The content of Mangnolia officinalis from Fengshan, Chadi, Liugou, Maliu and Aba was low, from other habitats were more than2.0%,conformed to the criterion of pharmacopoeia.The method was sensitive simple and accurate,multi-wavelength switching enforces the overall evaluation of Mangnolia officinalis from Sichuan Province and provided a suggestion for enhancing the Mangnolia officinalis from Sichuan province quality control.

关 键 词：四川厚朴 波长切换 高效液相色谱法指纹图谱 含量测定 质量控制 

分 类 号：O657.72[理学—分析化学] R284.1[理学—化学]