基于NGS技术的仔猪腹泻病毒检测  

The Detection of Diarrhea Virus in Piglet by NGS

作　　者：罗文涛 何启盖[2,3] 陈芳洲[2,3] 李静 周杰珑[1] Luo Wentao;He Qigai;Chen Fangzhou;Li Jing;Zhou Jielong(College of Life Sciences,Southwest Forestry Lniversity,Kunming Y unnan 650224,China;State Key Laboratory of Agricultural Microbiology,Huazhong Agricultural Lniversity,W＇uhan Hubei 430070,China;College of Animal Science and Veterinary Medicine,Huazhong Agricultural Lniversity,W uhan Hubei 430070,China)

机构地区：[1]西南林业大学生命科学学院,云南昆明650224 [2]华中农业大学农业微生物学国家重点实验室,湖北武汉430070 [3]华中农业大学动物医学院,湖北武汉430070

出　　处：《西南林业大学学报（自然科学）》2018年第4期112-118,共7页Journal of Southwest Forestry University：Natural Sciences

基　　金：云南省优势特色重点学科生物学一级学科研究生科技创新基金第二批(50097505)资助;云南省研发经费投入补助项目(2017YB236&2016YB462)资助

摘　　要：为探究四川某猪场反复暴发仔猪腹泻的原因,利用下一代测序技术(NGS)对该猪场的仔猪肠道样品进行检测,结合常规PCR、测序方法进行验证分析。结果表明:该猪场存在猪流行性腹泻病毒PEDV,通过对该场PEDV S1基因的进化树分析,发现该PEDV毒株属于独立的一个分支;同时还在该场病料中检测到猪圆环病毒3型(PCV3),该猪场腹泻问题可能是由于新型PEDV毒株和PCV3毒株混合感染导致。To explore the causes of repeated outbreaks of diarrhea in a pig farm in Sichuan. Utilizing next-generation sequencing technology （NGS） , combined with conventional PCR and sequencing methods to test and verify the pigletrs enteric intestinal samples. The results show the presence of porcine epidemic diarrhea virus （PEDV） exists in this farm. The PEDV strain was found to be a separate branch through phylogenetic tree analysis of the PEDV S1 gene. The porcine circovirus type 3 （PCV3） was also detected in the disease samples. This indicates that the diarrhea problem in the farm may be due to the co-infection of the new PEDV strain and the PCV3 strain.

关 键 词：NGS 猪 腹泻 病毒 PEDV PCV3 

分 类 号：S858.28[农业科学—临床兽医学]