人持续性G病毒Ⅱ型在献血者和反复接受输血患者中的检测与意义  

Detection of a novel human pegivirus HPgV-2 in healthy blood donors and recipients of multiple transfusions: implications for blood safety

作　　者：朱娜玲 许如 唐伟平 王海鹰 万政伟 吴学东[3] 付涌水[2] 唐时幸 俞守义 ZHU Naling;XU Ru;TANG Weiping;WANG Haiying;WAN Zhengwei;WU Xuedong;FU Yongshui;TANG Shixing;YU Shouyi(Guangdong Provincial Key Laboratory of Tropical Disease Research/Department of Epidemiology,School of Public Health,Southern Medical University,Guangzhou 510515,China;Institute of Blood Transfusion,Guangzhou Blood Center,Guangzhou 510000,China;Department of Pediatrics,Nanfang Hospital Southern Medical University,Guangzhou 510515,China)

机构地区：[1]南方医科大学公共卫生学院流行病系//热带病研究所,广东广州510515 [2]广州血液中心输血研究所,广东广州510000 [3]南方医科大学南方医院儿科,广东广州510515

出　　处：《南方医科大学学报》2018年第7期842-849,共8页Journal of Southern Medical University

基　　金：广州市科技计划项目(201604020011;201704020219)

摘　　要：目的确定我国献血者、反复接受输血患者HPgV-2感染率,阐明HPgV-2对血液安全的可能影响。方法运用血清学、反转录聚合酶链反应(RT-PCR)等方法分别在1060份健康献血者、1402份HCV、500份HBV、570份反复接受输血者、248份血友病患者共3780份血清标本中检测抗HPgV-2抗体和HPgV-2 RNA,对获得的HPgV-2 NS3片段和HPgV-2全基因组进行核酸序列测定和分子进化分析。同时检测献血者、反复接受输血者和血友病患者血清丙型肝炎病毒(HCV)和乙型肝炎病毒(HBV)感染情况辅助作为实验结果。结果(1)感染HCV(RNA+/Ab+)的献血者中抗HPgV-2抗体阳性率为1.21%(17/1402),核酸阳性率为0.36%(5/1402),而其他几组人群中几乎未检出HPgV-2病毒,这提示HPgV-2感染可能主要与HCV密切相关(χ2=13.78,P=0.004);(2)5株HPgV-2病毒保守区域NS3区同源性达97.11%,与国外分离毒株比较,其同源性为96.53%,未发现明显变异株。结论健康献血者中HPgV-2的感染率很低,HPgV-2主要和HCV共感染,反复接受输血患者和血友病患者没有发现HPgV-2感染,提示HPgV-2经输血传播不常见,可能对血液安全影响不明显。Objective To investigate the prevalence of HPgV-2 in blood donors, transfusion recipients and hemophilia patients and its impact on blood safety. Methods Serum samples were collected from 1060 healthy blood donors, 1402 HCV-positive and 500 HBV-positive blood donors, 570 transfusion recipients and 248 hemophilia patients for screening anti-HPgV-2 antibodies, HPgV-2 RNA, anti-HCV and HBs Ag/HBV-DNA using ELISA and RT-PCR. Phylogenetic analysis of near fulllength genome sequences and NS3 genes of pegiviruses and hepaciviruses were performed using MEGA software. Results Anti-HPgV-2 positivity and HPgV-2 RNA positivity were found in 1.21%（17/1402） and 0.36%（5/1402） of the blood donors infected with HCV（RNA＋/Ab＋）, respectively, indicating a close correlation between HPgV-2 and HCV infection（χ2=13.78, P=0.004）. Anti-HPgV-2 antibody was hardly detected in the other populations. A nucleotide identity as high as 97.11% was found in the NS3 fragments among the 5 isolated HPgV-2 strains, which had a nucleotide identity of 96.53% with the reported strains isolated out of China. Conclusion The prevalence of HPgV-2 infection is rather low in healthy blood donors and transfusion recipients. Coinfection with HCV is common in HPgV-2 infection, and no evidence has now been available to support HPgV-2 transmission via blood transfusion, indicating that HPgV-2 may not pose a threat to blood safety.

关 键 词：人持续性G病毒Ⅱ型 献血者 感染率 血液安全 

分 类 号：R446[医药卫生—诊断学]