HPLC法测定金银花炒炭前后芦丁等6种有效成分的含量变化  

Determination of Six Effective Ingredients in Honeysuckle before and after charred by HPLC

作　　者：邵琼[1] 张广琴[1] 严敢意 洪琳 王艳梅[1] SHAO Qiong;ZHANG Guangqin;YAN Ganyi(Department of Pharmacy,Ezhou Central Hospital（Ezhou Hubei 436000,China)

机构地区：[1]鄂州市中心医院药学部,湖北鄂州436000

出　　处：《四川中医》2018年第7期82-85,共4页Journal of Sichuan of Traditional Chinese Medicine

摘　　要：目的:探讨高效液相色谱法(HPLC)测定金银花炒炭前后芦丁等6种有效成分的含量变化。方法:取适量金银花药材,将其均分为6份,每份200g,270℃炒至焦褐色,炒炭细粉过筛后加入50%甲醇,超声1小时,采用0.45μm滤膜过滤后待检。同时制备对照品溶液。采用HPLC法,色谱柱为Aglient phenyl柱(250mm×4.6mm,5μm),以乙腈(A)-0.5%醋酸(B)溶液流动相进行梯度洗脱操作,以355nm为检测波长,柱温26℃,检测金银花炒炭前后芦丁、咖啡酸、绿原酸、槲皮素、木犀草苷、金丝桃苷等6种有效成分含量变化。结果:芦丁、咖啡酸、绿原酸、槲皮素、木犀草苷、金丝桃苷检测进样量均成良好线性关系,线性范围分别为:0.0022~0.83μg(r=0.9996)、0.0020~0.85μg(r=0.9997)、0.0014~9.73μg(r=0.9996)、0.0021~0.84μg(r=0.9995)、0.0024~0.87μg(r=0.9994)、0.0020~0.93μg(r=0.9998)。稳定性、精密性及重复性试验RSD<2.30%,稳定性、精密性及重复性良好。6种有效成分平均加样回收率分别为97.78%、97.76%、96.88%、98.31%、98.85%、99.54%。与炒炭前比较,炒炭后金银花中芦丁含量明显增加,咖啡酸、绿原酸含量明显下降,槲皮素、木犀草苷、金丝桃苷含量无明显变化。结论:金银花炒炭前后6种有效成分存在一定变化,HPLC法可有效检测金银花6种有效成分,且操作简单、稳定性、精密性及重复性好。Objective： To investigate the determination of six effective ingredients in Honeysuckle before and after charred by HPLC. Methods： Taking appropriate honeysuckle herbs,and divided into 6 copies,each 200 g,270℃ fry until coke brown,scrambled charcoal after screening by adding 50% methanol,ultrasound 1 h,0. 45μm filter to be seized. At the same time,prepared the reference solution. The column was Aglient phenyl column（ 250 mm×4. 6 mm,5μm）,the mobile phase was acetonitrile（ A）-0. 5% acetic acid（ B） solution,the detection wavelength was at 355 nm. The contents of rutin,caffeic acid,chlorogenic acid,quercetin and other 6 active ingredients were determined by HPLC. Results： Six kinds of active ingredients test injection volume into a good linear relationship. The linear range was 0. 0022 - 0. 83μg（ r = 0. 9996）,0. 0020 - 0. 85μg（ r = 0. 9997）,0. 0014 - 9. 73μg（ r = 0. 9996）,0. 0021 - 0. 84μg（ r = 0. 9995）,0. 0024 - 0. 87μg（ r = 0. 9994）,0. 0020 - 0. 93μg（ r =0. 9998）. Stability,precision and repeatability test RSD〈2. 30%,stability,precision and repeatability. The average recoveries of the six active ingredients were 97. 78%,97. 76%,96. 88%,98. 31%,98. 85% and 99. 54%. Compared with the former,the content of rutin in honeysuckle increased obviously,the content of caffeic acid and chlorogenic acid decreased obviously,the content of quercetin,luteolin and hyperoside did not change obviously. Conclusion： Before and after the honeysuckle charcoald,6 active ingredients there were a certain change,HPLC method can effectively detect six kinds of effective components of honeysuckle,and the simple operation,stability,precision and repeatability.

关 键 词：高效液相色谱法 炒炭 金银花 有效成分 变化 

分 类 号：R284.2[医药卫生—中药学]