变异冬红果组织培养中褐化控制研究  

Research about Controlling Browning of Mutant Malus spectabilis

作　　者：韦晨 丁悦来 王醒 WEI Chen;DING Yue-lai;WANG Xing(Key Laboratory of Modern Agricultural Equipment and Technology,Jiangsu University,Zhenjiang,Jiangsu 212013;Liyang Middle School of Jiangsu Province,Changzhou,Jiangsu 213357;Jiangsu Yixuan Landscape Engineer-ing Co.,Ltd.,Zhenjiang,Jiangsu 212000)

机构地区：[1]江苏大学,现代农业装备与技术教育部重点实验室,江苏镇江212013 [2]江苏省溧阳中学,江苏常州213357 [3]江苏艺轩园林景观工程有限公司,江苏镇江212000

出　　处：《安徽农业科学》2018年第17期102-104,共3页Journal of Anhui Agricultural Sciences

基　　金：镇江市重点研发计划-现代农业(NY2015013);江苏省农业科技自主创新项目[CX(15)1004]

摘　　要：[目的]研究不同因素对变异冬红果茎段褐化的影响。[方法]以变异冬红果茎段为外植体,研究茎段木质化程度、不同消毒方法、茎段转移频率、不同6-BA浓度、不同活性炭浓度对褐化的影响。[结果]同一灭菌处理下,木质化茎段的褐化率最高;单独用0.1%HgCl_2溶液消毒更有利于减轻变异冬红果茎段的褐化程度;转移频率为1 d时,更有利于减轻变异冬红果茎段的褐化程度;低浓度的6-BA有利于减轻变异冬红果茎段的褐化程度;在培养基中添加活性炭并不一定适合变异冬红果茎段的防褐化处理。[结论]该研究可为变异冬红果茎段的继代培养提供一定的参考。[Objective] To study the effect of different factors on the degree of browning in the stem segments of mutant Malus spectabilis.[Method]With mutant M. spectabilis stem segments as explant,to study the lignification degree of stem segments,different disinfection methods,transfer frequency of stem segments,different concentrations of 6-BA,different concentrations of activated carbon on the degree of browning. [Result] Under the same sterilization treatment,the browning rate of the lignified stem segments was the highest; disinfection with 0. 1%HgCl2 solution alone was more conducive to reduce the degree of browning of stem segments; when the transfer frequency was 1 d,it was more conducive to the reduction of browning of stem segments. Low concentration of 6-BA was beneficial to reduce the degree of browning of stem segments; adding activated carbon in the medium was not necessarily suitable for browning of stem segments of mutant M. spectabilis. [Conclusion] It provides some reference for the subculture of mutant M. spectabilis.

关 键 词：变异冬红果 组织培养 褐化 外植体 

分 类 号：S723.1[农业科学—林木遗传育种]