小麦HMW-GS分子检测中DNA提取方法的改良  

Improvement of DNA Extraction Methods in Wheat HMW-GS Molecular Detection

作　　者：张婷婷 王陆军[2,3] 郝建平 王艳梅[2,3] 王创云 李培良[2,3] 邓艳芳 周琼[3] 李志敏 庞冰[1] 董永军 裴成成 牛学谦 ZHANG Tingting;WANG Lujun;HAO Jianping;WANG Yanmei;WAN GChuangyun;LI Peiliang;DENG Yanfang;ZHOU Qiong;LI Zhimin;PANGBing;DONGYongjun;PEI Chengcheng;NIUXueqian(College of Life Science,Shanxi Univers ity,Taiyuan 030006,China;Ins titute of Crop Sciences,Shanxi Academy of AgriculturalSciences,Taiyuan 030031,China;Shanxi Corn Engineering Technology Research Center,Taiyuan 030031,China)

机构地区：[1]山西大学生命科学学院,山西太原030006 [2]山西省农业科学院作物科学研究所,山西太原030031 [3]山西省玉米工程技术研究中心,山西太原030031

出　　处：《山西农业科学》2018年第6期871-873,共3页Journal of Shanxi Agricultural Sciences

基　　金：山西省科技攻关项目(20140311001-8);山西省玉米工程技术研究中心建设项目(201605D141005);山西省重点研发计划项目(201703D221005-1)

摘　　要：为满足小麦高分子量谷蛋白亚基(HMW-GS)高通量分子检测的需求,以小麦种子为材料,以传统CTAB法为基础,结合磁珠法,创新了一种操作简便、成本低廉、周期短的小麦种子高通量DNA提取新方法,并采用紫外分光光度计、琼脂糖凝胶电泳、AS-PCR等方法验证了DNA的产率和质量。结果显示,采用新方法提取的基因组DNA产率和质量稳定,满足了小麦HMW-GS分子检测的要求。To meet the demand of high-throughput molecular detection of HMW-GS in wheat, based on the traditional CTAB method and magnetic bead method, this study created a new method of high-throughput DNA extraction of wheat seeds, which was simple operation, low cost, short cycle. The yield and quality of DNA were verified by ultraviolet spectrophotometer, agarose gel electrophoresis and AS-PCR. The results showed that the yield and quality of the genomic DNA extracted by the new method were stable,and met the requirement of wheat HMW-GS molecular detection.

关 键 词：小麦 种子 DNA CTAB 

分 类 号：S512.1[农业科学—作物学]